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Literature summary extracted from

  • Gomez-Manzo, S.; Contreras-Zentella, M.; Gonzalez-Valdez, A.; Sosa-Torres, M.; Arreguin-Espinoza, R.; Escamilla-Marvan, E.
    The PQQ-alcohol dehydrogenase of Gluconacetobacter diazotrophicus (2008), Int. J. Food Microbiol., 125, 71-78.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.5 membrane bound Gluconacetobacter diazotrophicus 16020
-
1.2.5.2 membrane bound Gluconacetobacter diazotrophicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.5 43500
-
 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
1.1.5.5 71400
-
 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
1.1.5.5 115000
-
 non-denaturing PAGE Gluconacetobacter diazotrophicus
1.2.5.2 43500
-
 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
1.2.5.2 71400
-
 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
1.2.5.2 115000
-
 non-denaturing PAGE Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.5.2 acetaldehyde + ubiquinone Gluconacetobacter diazotrophicus electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone acetate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.5 Gluconacetobacter diazotrophicus
-
-
-
1.2.5.2 Gluconacetobacter diazotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.5
-
 Gluconacetobacter diazotrophicus
1.2.5.2
-
 Gluconacetobacter diazotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.5 258
-
-
 Gluconacetobacter diazotrophicus
1.2.5.2 258
-
-
 Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.5 allyl alcohol + ferricyanide the best substrate Gluconacetobacter diazotrophicus acrolein + ferricyanide
-
 ?
1.1.5.5 ethanol + ferricyanide 95% of the activity with allyl alcohol Gluconacetobacter diazotrophicus acetaldehyde + ferrocyanide
-
 ?
1.1.5.5 isopropanol + ferricyanide 18% of the activity with allyl alcohol Gluconacetobacter diazotrophicus propan-2-one + ferrocyanide
-
 ?
1.1.5.5 methanol + ferricyanide 9% of the activity with allyl alcohol Gluconacetobacter diazotrophicus formaldehyde + ferrocyanide
-
 ?
1.1.5.5 additional information no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol Gluconacetobacter diazotrophicus ?
-
 ?
1.1.5.5 n-butanol + ferricyanide 98% of the activity with allyl alcohol Gluconacetobacter diazotrophicus butyraldehyde + ferrocyanide
-
 ?
1.1.5.5 n-propanol + ferricyanide 90% of the activity with allyl alcohol Gluconacetobacter diazotrophicus propionaldehyde + ferrocyanide
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O 87% of the activity with allyl alcohol Gluconacetobacter diazotrophicus acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + ubiquinone electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone Gluconacetobacter diazotrophicus acetate + ubiquinol
-
 ?
1.2.5.2 glutaraldehyde + 4 ferricyanide + 2 H2O 25% of the activity with allyl alcohol Gluconacetobacter diazotrophicus glutarate + 4 ferrocyanide + 4 H+
-
 ?
1.2.5.2 additional information no activity with glucose, benzaldehyde, formaldehyde, acetone, sorbitol or glycerol Gluconacetobacter diazotrophicus ?
-
 ?
1.2.5.2 propanal + 2 ferricyanide + H2O 42% of the activity with allyl alcohol Gluconacetobacter diazotrophicus propanoate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.5 dimer 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus
1.2.5.2 dimer 1 * 71400 + 1 * 43500, SDS-PAGE Gluconacetobacter diazotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 PQQ-alcohol dehydrogenase
-
 Gluconacetobacter diazotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.5 40
-
 15 min, 80% loss of activity Gluconacetobacter diazotrophicus
1.2.5.2 40
-
 15 min, 80% loss of activity Gluconacetobacter diazotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.5 6
-
 substrate: ethanol Gluconacetobacter diazotrophicus
1.2.5.2 6
-
 substrate: acetaldehyde Gluconacetobacter diazotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.5 5 7.5 pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: ethanol Gluconacetobacter diazotrophicus
1.2.5.2 5 7.5 pH 5.0: about 50% of maximal activity, pH 7.5: about 55% of maximal activity, substrate: acetaldehyde Gluconacetobacter diazotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.5 cytochrome c presence of cytochrome c in both subunits Gluconacetobacter diazotrophicus
1.1.5.5 pyrroloquinoline quinone electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c Gluconacetobacter diazotrophicus
1.2.5.2 cytochrome c presence of cytochrome c in both subunits Gluconacetobacter diazotrophicus
1.2.5.2 pyrroloquinoline quinone electrons removed from substrate by alcohol dehydrogenase complex are initially transferred to the pyrroloquinoline quinone centre and further tunnelled across four cytochromes c to the membrane ubiquinone Gluconacetobacter diazotrophicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.5.5 Gluconacetobacter diazotrophicus gradient electrophoresis, determined in pH range 3.4-9.0
-
 6.1
1.2.5.2 Gluconacetobacter diazotrophicus gradient electrophoresis, determined in pH range 3.4-9.0
-
 6.1