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Literature summary extracted from

  • Mohammadi, H.S.; Omidinia, E.; Lotfi, A.S.; Saghiri, R.
    Preliminary report of NAD+-dependent amino acid dehydrogenase producing bacteria isolated from soil (2007), Iran. Biomed. J., 11, 131-135.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.5 0.012
-
 L-glutamine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Proteus sp.
1.4.1.5 0.025
-
 L-isoleucine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Citrobacter sp.
1.4.1.5 0.034
-
 L-methionine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Proteus sp.
1.4.1.5 0.044
-
 L-valine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.5 Citrobacter sp.
-
-
-
1.4.1.5 Proteus sp.
-
-
-
1.4.1.5 Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.5 0.47
-
 using L-valine, L-cysteine, L-serine, or L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) Pseudomonas sp.
1.4.1.5 0.7
-
 using L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) Citrobacter sp.
1.4.1.5 0.73
-
 using L-cysteine, L-serine, or L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) Proteus sp.
1.4.1.5 1.2
-
 using L-methionine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) Proteus sp.
1.4.1.5 1.59
-
 using L-isoleucine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) Citrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.5 L-cysteine + H2O + NAD+
-
 Pseudomonas sp. 3-mercapto-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-cysteine + H2O + NAD+
-
 Citrobacter sp. 3-mercapto-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-cysteine + H2O + NAD+
-
 Proteus sp. 3-mercapto-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-glutamine + H2O + NAD+
-
 Pseudomonas sp. 2-oxoglutaramate + NH3 + NADH
-
 ?
1.4.1.5 L-glutamine + H2O + NAD+
-
 Citrobacter sp. 2-oxoglutaramate + NH3 + NADH
-
 ?
1.4.1.5 L-glutamine + H2O + NAD+
-
 Proteus sp. 2-oxoglutaramate + NH3 + NADH
-
 ?
1.4.1.5 L-isoleucine + H2O + NAD+ best substrate Citrobacter sp. 3-methyl-2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.5 L-methionine + H2O + NAD+
-
 Proteus sp. (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.5 L-serine + H2O + NAD+
-
 Pseudomonas sp. 3-hydroxy-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-serine + H2O + NAD+
-
 Citrobacter sp. 3-hydroxy-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-serine + H2O + NAD+
-
 Proteus sp. 3-hydroxy-2-oxopropanoate + NH3 + NADH
-
 ?
1.4.1.5 L-valine + H2O + NAD+ highest activity Pseudomonas sp. 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.5 additional information no amino acid dehydrogenase activity for L-asparagine Proteus sp. ?
-
 ?
1.4.1.5 additional information no amino acid dehydrogenase activity for L-isoleucine amino acid analogs including L-leucine, L-valine, L-alanine or L-methionine Citrobacter sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.5 NAD+-dependent amino acid dehydrogenase
-
 Pseudomonas sp.
1.4.1.5 NAD+-dependent amino acid dehydrogenase
-
 Citrobacter sp.
1.4.1.5 NAD+-dependent amino acid dehydrogenase
-
 Proteus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.5 1.15
-
 L-glutamine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Proteus sp.
1.4.1.5 2.11
-
 L-methionine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Proteus sp.
1.4.1.5 2.84
-
 L-isoleucine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Citrobacter sp.
1.4.1.5 4.95
-
 L-valine in 100 mM glycine-KCl-KOH buffer (pH 10.4) Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.5 NAD+ dependent Pseudomonas sp.
1.4.1.5 NAD+ dependent Citrobacter sp.
1.4.1.5 NAD+ dependent Proteus sp.