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Literature summary extracted from

  • van Haaster, D.J.; Silva, P.J.; Hagedoorn, P.L.; Jongejan, J.A.; Hagen, W.R.
    Reinvestigation of the steady-state kinetics and physiological function of the soluble NiFe-hydrogenase I of Pyrococcus furiosus (2008), J. Bacteriol., 190, 1584-1587.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.1.3 CO 0.02 mM, 50% inhibition Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.1.3 0.018
-
 H2 cosubstrate: benzyl viologen Pyrococcus furiosus
1.12.1.3 0.02
-
 H2 cosubstrate: methyl viologen Pyrococcus furiosus
1.12.1.3 0.037
-
 NADP+
-
 Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.3 soluble
-
 Pyrococcus furiosus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.3 H2 + NADP+ Pyrococcus furiosus the enzyme likely functions in the regeneration of NADPH and thus reuses the hydrogen produced by the membrane-bound hydrogenase in proton respiration H+ + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.3 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.3 H2 + NADP+ the enzyme likely functions in the regeneration of NADPH and thus reuses the hydrogen produced by the membrane-bound hydrogenase in proton respiration Pyrococcus furiosus H+ + NADPH
-
 r
1.12.1.3 H2 + NADP+ the hydrogen consumption activity is 1 order of magnitude higher than the hydrogen production activity Pyrococcus furiosus H+ + NADPH
-
 r
1.12.1.3 H2 + oxidized benzyl viologen
-
 Pyrococcus furiosus H+ + reduced benzyl viologen
-
 ?
1.12.1.3 H2 + oxidized methyl viologen
-
 Pyrococcus furiosus H+ + reduced methyl viologen
-
 ?