Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chang, Y.; Wesenberg, G.; Bingman, C.A.; Fox, B.G.
    In vivo inactivation of mycobacterial integral membrane stearoyl-CoA desaturase DesA3 by a C-terminal specific degradation process (2008), J. Bacteriol., 190, 6686-6696.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.1 gene rv3229c, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli as c-Myc and/or His-tagged protein, DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA. The native C-terminal sequence of LAA apparently serves as a binding determinant for a mycobacterial protease/degradation system directed at DesA3. Fusion of only the last 12 residues of native DesA3 to the C-terminal of GFP is sufficient to make GFP unstable Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.1 additional information stability and activity of several GFP-fusion mutant enzymes variants, resistance os sensitivity to mycobacterial proteases, overview Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.1 membrane integral Mycobacterium tuberculosis 16020
-
1.14.19.1 microsome
-
 Mycobacterium tuberculosis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.1 75000
-
 x * 75000, about, recombinant untagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 additional information Mycobacterium tuberculosis DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides ?
-
 ?
1.14.19.1 additional information Mycobacterium tuberculosis H37Rv DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides ?
-
 ?
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ Mycobacterium tuberculosis essential enzyme activity, regulation mechanism, overview oleoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ Mycobacterium tuberculosis H37Rv essential enzyme activity, regulation mechanism, overview oleoyl-CoA + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Mycobacterium tuberculosis
-
 gene rv3229c
-
1.14.19.1 Mycobacterium tuberculosis H37Rv
-
 gene rv3229c
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 additional information DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides Mycobacterium tuberculosis ?
-
 ?
1.14.19.1 additional information the enzyme reacts with oxidoreductase Rv3230c to produce oleic acid Mycobacterium tuberculosis ?
-
 ?
1.14.19.1 additional information DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides Mycobacterium tuberculosis H37Rv ?
-
 ?
1.14.19.1 additional information the enzyme reacts with oxidoreductase Rv3230c to produce oleic acid Mycobacterium tuberculosis H37Rv ?
-
 ?
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ essential enzyme activity, regulation mechanism, overview Mycobacterium tuberculosis oleoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA Mycobacterium tuberculosis oleoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ essential enzyme activity, regulation mechanism, overview Mycobacterium tuberculosis H37Rv oleoyl-CoA + ferricytochrome b5 + H2O
-
 ?
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA Mycobacterium tuberculosis H37Rv oleoyl-CoA + ferricytochrome b5 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.1 ? x * 75000, about, recombinant untagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.1 DesA3
-
 Mycobacterium tuberculosis
1.14.19.1 membrane-bound stearoyl-CoA DELTA9 desaturase
-
 Mycobacterium tuberculosis
1.14.19.1 stearoyl-CoA desaturase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 ferrocytochrome b5
-
 Mycobacterium tuberculosis