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Literature summary extracted from

  • Szeto, S.S.; Reinke, S.N.; Sykes, B.D.; Lemire, B.D.
    Ubiquinone-binding site mutations in the Saccharomyces cerevisiae succinate dehydrogenase generate superoxide and lead to the accumulation of succinate (2007), J. Biol. Chem., 282, 27518-27526.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.5.1 phenazine methosulfate
-
Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.1 genes SDHA, SDHB, SDHC, and SDHD Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.1 D88E site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro Saccharomyces cerevisiae
1.3.5.1 D88K site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content Saccharomyces cerevisiae
1.3.5.1 D88N site-directed mutagenesis of a subunit Sdh4p residue, the mutant shows reduced activity and FAD content, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro Saccharomyces cerevisiae
1.3.5.1 additional information ubiquinone-binding site mutations in the succinate dehydrogenase generate superoxide and lead to the accumulation of succinate Saccharomyces cerevisiae
1.3.5.1 R47C site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity Saccharomyces cerevisiae
1.3.5.1 R47E site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity and FAD content Saccharomyces cerevisiae
1.3.5.1 R47K site-directed mutagenesis of a subunit Sdh3p residue, the mutant shows reduced activity, as well as accumulation and secretion of succinate, the mutant is sensitive to hyperoxia and paraquate and shows enhanced superoxide production in vivo and in vitro Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 malonate
-
Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 mitochondrial membrane
-
Saccharomyces cerevisiae 31966
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.1 succinate + ubiquinone Saccharomyces cerevisiae SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane fumarate + ubiquinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Saccharomyces cerevisiae
-
genes SDHA, SDHB, SDHC, and SDHD encding the four subunits of the enzyme
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.1 additional information
-
substrate specificities of wild-type and mutant enzymes, overview Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + decylubiquinone decylubiquinone-mediated reduction of dichlorophenol indophenol Saccharomyces cerevisiae fumarate + decylubiquinol
-
?
1.3.5.1 succinate + oxidized 2,6-dichlorophenolindophenol succinate-dependent, phenazine methosulfate-mediated malonate-sensitive reduction of 2,6-dichlorophenol indophenol Saccharomyces cerevisiae fumarate + reduced 2,6-dichlorophenolindophenol
-
?
1.3.5.1 succinate + ubiquinone SDH is an essential component of the electron transport chain and of the tricarboxylic acid cycle in the mitochondrial membrane Saccharomyces cerevisiae fumarate + ubiquinol
-
?

Subunits

EC Number Subunits Comment Organism
1.3.5.1 tetramer subunits SdhA, SdhB, SdhC, and SdhD Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 mitochondrial succinate dehydrogenase
-
Saccharomyces cerevisiae
1.3.5.1 SDH
-
Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 2,6-dichlorophenolindophenol
-
Saccharomyces cerevisiae
1.3.5.1 decylubiquinone
-
Saccharomyces cerevisiae
1.3.5.1 FAD
-
Saccharomyces cerevisiae
1.3.5.1 ubiquinone
-
Saccharomyces cerevisiae