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Literature summary extracted from

  • Vogt, S.; Lyon, E.J.; Shima, S.; Thauer, R.K.
    The exchange activities of [Fe] hydrogenase (iron-sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases (2008), J. Biol. Inorg. Chem., 13, 97-106.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 expressed in Escherichia coli BL21 (DE3) cells Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.99.6 0.01
-
 methylenetetrahydromethanopterin at pH 6.0 and 25°C Methanocaldococcus jannaschii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 cytoplasm
-
 Methanocaldococcus jannaschii 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Iron the enzyme harbors an iron-containing cofactor, in which a lowspin iron is complexed by a pyridone, two CO and a cysteine sulfur, [Fe] hydrogenase apoenzyme is converted completely into [Fe] hydrogenase holoenzyme by mixing the apoenzyme with a 3fold excess of the iron-containing [Fe] hydrogenase cofactor Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 38000
-
 2 * 38000, SDS-PAGE Methanocaldococcus jannaschii
1.12.99.6 76000
-
 SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 ammonium sulfate precipitation and phenyl-Sepharose column chromatography Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 150
-
 at pH 6.0 and 25°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + methenyltetrahydromethanopterin the presence of methenyltetrahydromethanopterin is essentially required for H2 activation by [Fe] hydrogenase Methanocaldococcus jannaschii H+ + methylenetetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.99.6 homodimer 2 * 38000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 Hmd
-
 Methanocaldococcus jannaschii
1.12.99.6 hydrogen-forming methylenetetrahydromethanopterin dehydrogenase
-
 Methanocaldococcus jannaschii
1.12.99.6 iron-sulfur-cluster-free hydrogenase
-
 Methanocaldococcus jannaschii
1.12.99.6 [Fe] hydrogenase
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.99.6 65
-
 the enzyme is rapidly inactivated at 65°C Methanocaldococcus jannaschii