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Literature summary extracted from

  • Cho, H.Y.; Son, S.Y.; Rhee, H.S.; Yoon, S.Y.; Lee-Parsons, C.W.; Park, J.M.
    Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures (2008), J. Biotechnol., 135, 117-122.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.102 additional information sequential treatment with methyl jasmonate, salicylic acid, and yeast extract leads to accumulation of benzophenanthridine alkaloids and increased expression of involved synthesizing enzymes, sequential treatment using different signal elicitors is more effective than single elicitor or simultaneous treatment with the elicitors, overview Eschscholzia californica
2.1.1.116 methyl jasmonate 4'OMT is rapidly induced with high levels of expression at 6-12 h after methyl jasmonate treatment Eschscholzia californica
2.1.1.116 salicylic acid salicylic acid induces a delayed accumulation of 4'OMT Eschscholzia californica
2.1.1.116 yeast extract yeast extract induces a delayed accumulation of 4'OMT Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 Eschscholzia californica the enzyme is involved in the sanguinarine biosynthetic pathway, overview (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine Eschscholzia californica
-
 S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.102 Eschscholzia californica
-
-
-
2.1.1.116 Eschscholzia californica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.116 Eschscholtzia californica cells are frozen using liquid nitrogen and suspended in protein extraction buffer, the supernatant is collected after centrifugation, protein samples are fractionated by SDS-PAGE Eschscholzia californica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.102 cell suspension culture
-
 Eschscholzia californica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
 Eschscholzia californica (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in the sanguinarine biosynthetic pathway, overview Eschscholzia californica (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 Eschscholzia californica S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.102 (S)-methylcoclaurine-3'-hydroxylase
-
 Eschscholzia californica
1.14.14.102 CYP80B1
-
 Eschscholzia californica
2.1.1.116 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase
-
 Eschscholzia californica
2.1.1.116 4'OMT
-
 Eschscholzia californica