EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.79 | expressed as GFP-fusion protein in tobacco BY-2 cells | Arabidopsis thaliana |
1.1.1.79 | expressed as His-tag fusion protein in E. coli BL-21(DE3) Rosetta (pLysS), full-length and truncated GR2 sequences introduced into Escherichia coli, only the recombinant truncated GR2 is soluble, expression markedly improved by co-expression of the GroES/GroEL chaperone | Arabidopsis thaliana |
1.1.1.79 | expressed in Escherichia coli BL-21(DE3) Rosetta (pLysS) cells and in Nicotiana tabacum BY-2 cells | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.79 | DELTA1-43 | in contrast to the full length sequence yields a soluble protein when expressed in Escherichia coli | Arabidopsis thaliana |
1.1.1.79 | DELTA2-45 | localizes in the cytosol instead of plastids | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.79 | NADP+ | competitive | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.79 | 0.0012 | - |
NADPH | with succinic semialdehyde as substrate, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 0.0012 | - |
NADPH | recombinant enzyme, using succinic semialdehyde as fixed substrate, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana | |
1.1.1.79 | 0.0014 | - |
NADPH | with glyoxylate as substrate, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 0.0014 | - |
NADPH | recombinant enzyme, using glyoxylate as fixed substrate, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana | |
1.1.1.79 | 0.034 | - |
glyoxylate | with as NADPH as cofactor, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 0.034 | - |
glyoxylate | recombinant enzyme, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana | |
1.1.1.79 | 8.96 | - |
Succinic semialdehyde | with as NADPH as cofactor, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 8.96 | - |
Succinic semialdehyde | recombinant enzyme, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.79 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
1.1.1.79 | plastid | stroma | Arabidopsis thaliana | 9536 | - |
1.1.1.79 | plastid | glyoxylate reductase 2 is localized within the plastid, presumably in the stroma | Arabidopsis thaliana | 9536 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.79 | 35900 | - |
x * 35900, truncated enzyme from Escherichia coli including His-tag | Arabidopsis thaliana |
1.1.1.79 | 36000 | - |
SDS-PAGE | Arabidopsis thaliana |
1.1.1.79 | 36200 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
1.1.1.79 | 36287 | - |
x * 36287, calculated from the deduced amino acid sequence | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.79 | glyoxylate + NADPH + H+ | Arabidopsis thaliana | detoxification of glyoxylate during stress | glycolate + NADP+ | - |
ir | |
1.1.1.79 | succinic semialdehyde + NADPH + H+ | Arabidopsis thaliana | detoxification of succinic semialdehyde during stress | 4-hydroxybutyrate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.79 | Arabidopsis thaliana | F4I907 | ecotype Columbia | - |
1.1.1.79 | Arabidopsis thaliana | F4I907 | putative uncharacterized protein At1g17650; ecotype Columbia | - |
1.1.1.79 | Arabidopsis thaliana | Q9LSV0 | enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency; (L.) Heynh | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.79 | nickel affinity column chromatography | Arabidopsis thaliana |
1.1.1.79 | recombinant truncated protein using His-tag | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.79 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.79 | -80°C, concentrated enzyme, can be stored for month | Arabidopsis thaliana |
1.1.1.79 | -80°C, concentrated protein eluate from the affinity column, for months, enzyme activity is reasonably stable | Arabidopsis thaliana |
1.1.1.79 | 4°C, concentrated protein eluate from the affinity column, for hours, enzyme activity is reasonably stable | Arabidopsis thaliana |
1.1.1.79 | 4°C, diluted enzyme, storage during assays, activity starts to decrease within 30 min | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.79 | glyoxylate + NADH | NADH much less effective than NADPH | Arabidopsis thaliana | glycolate + NAD+ | - |
ir | |
1.1.1.79 | glyoxylate + NADH + H+ | NADH much less effective than NADPH | Arabidopsis thaliana | glycolate + NAD+ | - |
ir | |
1.1.1.79 | glyoxylate + NADPH + H+ | - |
Arabidopsis thaliana | glycolate + NADP+ | - |
ir | |
1.1.1.79 | glyoxylate + NADPH + H+ | detoxification of glyoxylate during stress | Arabidopsis thaliana | glycolate + NADP+ | - |
ir | |
1.1.1.79 | glyoxylate + NADPH + H+ | glyoxylate reductase 2 has a 350fold higher preference for glyoxylate than for succinic semialdehyde | Arabidopsis thaliana | glycolate + NADP+ | - |
ir | |
1.1.1.79 | additional information | glyoxylate reductase 2 is ineffective in catalysing the reverse reaction utilizing either glycolate or 6-phosphogluconate | Arabidopsis thaliana | ? | - |
? | |
1.1.1.79 | succinic semialdehyde + NADH + H+ | NADH much less effective than NADPH | Arabidopsis thaliana | 4-hydroxybutyrate + NAD+ | - |
? | |
1.1.1.79 | succinic semialdehyde + NADPH + H+ | - |
Arabidopsis thaliana | 4-hydroxybutyrate + NADP+ | - |
r | |
1.1.1.79 | succinic semialdehyde + NADPH + H+ | detoxification of succinic semialdehyde during stress | Arabidopsis thaliana | 4-hydroxybutyrate + NADP+ | - |
r | |
1.1.1.79 | succinic semialdehyde + NADPH + H+ | reverse reaction less efficient than forward reaction | Arabidopsis thaliana | 4-hydroxybutyrate + NADP+ | - |
r | |
1.1.1.79 | succinic semialdehyde + NADPH + H+ | glyoxylate reductase 2 has a 350fold higher preference for glyoxylate than for succinic semialdehyde | Arabidopsis thaliana | 4-hydroxybutyrate + NADP+ | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.79 | ? | x * 35900, truncated enzyme from Escherichia coli including His-tag | Arabidopsis thaliana |
1.1.1.79 | ? | x * 36287, calculated from the deduced amino acid sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.79 | AtGR1 | - |
Arabidopsis thaliana |
1.1.1.79 | AtGR2 | - |
Arabidopsis thaliana |
1.1.1.79 | glyoxylate reductase 1 | - |
Arabidopsis thaliana |
1.1.1.79 | glyoxylate reductase 2 | - |
Arabidopsis thaliana |
1.1.1.79 | GR2 | - |
Arabidopsis thaliana |
1.1.1.79 | More | enzyme also displays 4-hydroxybutyrate dehydrogenase (EC1.1.1.61) activity but with less efficiency | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.79 | 10.7 | - |
NADPH | with succinic semialdehyde as substrate, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 10.7 | - |
NADPH | recombinant enzyme, using succinic semialdehyde as fixed substrate, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana | |
1.1.1.79 | 12 | - |
NADPH | with glyoxylate as substrate, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 12 | - |
NADPH | recombinant enzyme, using glyoxylate as fixed substrate, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana | |
1.1.1.79 | 17 | - |
Succinic semialdehyde | with as NADPH as cofactor, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 17 | - |
Succinic semialdehyde | recombinant enzyme, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana | |
1.1.1.79 | 22.5 | - |
glyoxylate | with as NADPH as cofactor, pH 7.6, 30°C | Arabidopsis thaliana | |
1.1.1.79 | 22.5 | - |
glyoxylate | recombinant enzyme, in 50 mM HEPES (pH 7.6), at 30°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.79 | 6.8 | 7.8 | - |
Arabidopsis thaliana |
1.1.1.79 | 6.8 | 7.8 | with glyoxylate and NADPH | Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.79 | 5.5 | 9 | approximately 10% of activity at pH 5.5 and 9 | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.79 | NADH | much less effective than NADPH | Arabidopsis thaliana | |
1.1.1.79 | NADPH | - |
Arabidopsis thaliana | |
1.1.1.79 | NADPH | glyoxylate reductase 2 uses either NADPH or NADH as a cofactor, however, much greater activity is found with NADPH | Arabidopsis thaliana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.79 | Arabidopsis thaliana | calculated from amino acid sequence | - |
8.5 |
1.1.1.79 | Arabidopsis thaliana | calculated from the deduced amino acid sequence | - |
8.54 |