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Literature summary extracted from

  • Lee, Y.; Yeom, J.; Kang, Y.S.; Kim, J.; Sung, J.S.; Jeon, C.O.; Park, W.
    Molecular characterization of fprB (ferredoxin-NADP+ reductase) in Pseudomonas putida KT2440 (2007), J. Microbiol. Biotechnol., 17, 1504-1512.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.18.1.2 NaCl 0.51 M and 0.85 M NaCl increases Fpr expression Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.2 additional information induction of FprB expression is not affected by oxidative stress agents, such as paraquat, menadione, H2O2, and t-butyl hydroperoxide Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 28600
-
 calculated from amino acid sequence Pseudomonas putida
1.18.1.2 29000
-
 SDS-PAGE, purified enzyme Pseudomonas putida
1.18.1.2 35000
-
 Sephacryl S-200 gel filtration, native enzyme Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Pseudomonas putida
-
-
-
1.18.1.2 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 DEAE-Sepharose column chromatography and Sephacryl S-300 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 additional information Fpr also catalyzes irreversible electron transfer (diaphorase activity), which drives the oxidation of NADPH in a wide variety of electron acceptors, such as viologens, quinines, complexed transition metals, and tetrazolium salts Pseudomonas putida ?
-
 ?
1.18.1.2 additional information Fpr also catalyzes irreversible electron transfer (diaphorase activity), which drives the oxidation of NADPH in a wide variety of electron acceptors, such as viologens, quinines, complexed transition metals, and tetrazolium salts Pseudomonas putida KT 2240 ?
-
 ?
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase reaction Pseudomonas putida NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ir
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase reaction Pseudomonas putida KT 2240 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ir
1.18.1.2 reduced ferredoxin + NADP+
-
 Pseudomonas putida oxidized ferredoxin + NADPH
-
 r
1.18.1.2 reduced ferredoxin + NADP+
-
 Pseudomonas putida KT 2240 oxidized ferredoxin + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 35000, Sephacryl S-200 gel filtration, native enzyme Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
 Pseudomonas putida
1.18.1.2 FprB
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 NADP+
-
 Pseudomonas putida