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Literature summary extracted from

  • Fedoy, A.E.; Yang, N.; Martinez, A.; Leiros, H.K.; Steen, I.H.
    Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability (2007), J. Mol. Biol., 372, 130-149.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.42 crystals of native DpIDH are obtained by the sitting-drop, vapour-diffusion method. The crystals are grown at 8°C. Crystals of DpIDH in complex with isocitrate (DpIDH-iso) are obtained by the hanging drop method at 8 °C Desulfotalea psychrophila

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Desulfotalea psychrophila Q6AQ66
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
Desulfotalea psychrophila 2-oxoglutarate + CO2 + NADPH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 DpIDH
-
Desulfotalea psychrophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 70
-
melting temperature is 66.9°C Desulfotalea psychrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 4.5
-
NADP+ 5°C Desulfotalea psychrophila
1.1.1.42 4.5
-
isocitrate 5°C Desulfotalea psychrophila
1.1.1.42 31.4
-
NADP+ 25°C Desulfotalea psychrophila
1.1.1.42 31.4
-
isocitrate 25°C Desulfotalea psychrophila