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Literature summary extracted from

  • Loening, A.M.; Fenn, T.D.; Gambhir, S.S.
    Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis (2007), J. Mol. Biol., 374, 1017-1028.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression of stabilized variant RLuc8 with noncleavable N-terminal His6-tag Renilla reniformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.12.5 free enzyme and in complex with coelenterazine,and mutant K25A/E277A. Diffraction to 1.4 A. Structures demonstrate a classic alpha/beta-hydrolase fold. The presumptive catalytic triad residues are D120, E144, and H285. Additionally determination of structure of the accessory green fluorescent protein Renilla reniformis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 K25A/E277A surface mutations made with the intention that they would aid in crystallization, not involved in contacts between proteins in the crystal Renilla reniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.5 33800
-
 gel filtration Renilla reniformis
1.13.12.5 36800
-
 1 * 36800, mass spectrometry, 1 * 36900, calculated Renilla reniformis
1.13.12.5 36900
-
 1 * 36800, mass spectrometry, 1 * 36900, calculated Renilla reniformis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla reniformis P27652 variant RLuc8
-

Subunits

EC Number Subunits Comment Organism
1.13.12.5 monomer 1 * 36800, mass spectrometry, 1 * 36900, calculated Renilla reniformis