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Literature summary extracted from

  • Frick, S.; Kramell, R.; Kutchan, T.M.
    Metabolic engineering with a morphine biosynthetic P450 in opium poppy surpasses breeding (2007), Metab. Eng., 9, 169-176.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.102 biotechnology the enzyme provides practical means to genetically engineer valuable secondary metabolites in this important medicinal plant Papaver somniferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.102 gene cyp80b3, overexpression in Papaver somniferum plants, expression of anti-sense constructs, construction of transgenic plants using the Agrobacterium tumefaciens, strain AGL1, transfection method, expression of antisense cyp80b3 leads to a reduction of total alkaloid in latex up to 84%, phenotype, overview, enzyme overexpression results in an up to 450% increase in the amount of total alkaloid in latex without changing the ratio of the individual alkaloids, or together with an overall increase in the ratio of morphine Papaver somniferum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.102 Fe2+ a cytochrome P450 enzyme Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum the enzyme catalyzes a key regulation step in morphine biosynthesis (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.102 Papaver somniferum
-
 cv. C048-6-14-64
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.102 (S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme catalyzes a key regulation step in morphine biosynthesis Papaver somniferum (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.102 (S)-N-methylcoclaurine 3'-hydroxylase
-
 Papaver somniferum
1.14.14.102 CYP80B3
-
 Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.102 cytochrome P450 dependent on Papaver somniferum