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Literature summary extracted from

  • Portal, P.; Villamil, S.F.; Alonso, G.D.; De Vas, M.G.; Flawia, M.M.; Torres, H.N.; Paveto, C.
    Multiple NADPH-cytochrome P450 reductases from Trypanosoma cruzi suggested role on drug resistance (2008), Mol. Biochem. Parasitol., 160, 42-51.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 expression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 diphenyleneiodonium 0.03 mM, 62% inhibition; 0.03 mM, 64% inhibition; 0.03 mM, 76% inhibition Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.00577
-
 NADPH pH 7.7, 30°C Trypanosoma cruzi
1.6.2.4 0.0098
-
 ferricytochrome c pH 7.7, 30°C Trypanosoma cruzi
1.6.2.4 0.017
-
 ferricytochrome c pH 7.7, 30°C Trypanosoma cruzi
1.6.2.4 0.018
-
 ferricytochrome c pH 7.7, 30°C Trypanosoma cruzi
1.6.2.4 0.0511
-
 NADPH pH 7.7, 30°C Trypanosoma cruzi
1.6.2.4 0.05488
-
 NADPH pH 7.7, 30°C Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 68600
-
 x * 68600, calculated from sequence Trypanosoma cruzi
1.6.2.4 71300
-
 x * 71300, calculated from sequence Trypanosoma cruzi
1.6.2.4 78400
-
 x * 78400, calculated from sequence Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Trypanosoma cruzi Q003G7
-
-
1.6.2.4 Trypanosoma cruzi Q003G8
-
-
1.6.2.4 Trypanosoma cruzi Q0MRD4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4
-
 Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 amastigote
-
 Trypanosoma cruzi
-
1.6.2.4 epimastigote
-
 Trypanosoma cruzi
-
1.6.2.4 epimastigote overexpression of TcCPR-B in Trypanosoma cruzi epimastigotes increases its resistance to the typical chemotherapeutic agents Nifurtimox and Benznidazole Trypanosoma cruzi
-
1.6.2.4 additional information presence in every Trypanosoma cruzi developmental stage Trypanosoma cruzi
-
1.6.2.4 trypomastigote
-
 Trypanosoma cruzi
-
1.6.2.4 trypomastigote strongly expressed Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
 Trypanosoma cruzi 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 additional information menadione or adrenaline are not reduced Trypanosoma cruzi ?
-
 ?
1.6.2.4 additional information menadione or adrenaline ate not reduced Trypanosoma cruzi ?
-
 ?
1.6.2.4 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Trypanosoma cruzi NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 68600, calculated from sequence Trypanosoma cruzi
1.6.2.4 ? x * 71300, calculated from sequence Trypanosoma cruzi
1.6.2.4 ? x * 78400, calculated from sequence Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Trypanosoma cruzi
1.6.2.4 NADPH-cytochrome P450 reductase
-
 Trypanosoma cruzi
1.6.2.4 TcCPR-A
-
 Trypanosoma cruzi
1.6.2.4 TcCPR-B
-
 Trypanosoma cruzi
1.6.2.4 TcCPR-C
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Trypanosoma cruzi
1.6.2.4 ferricytochrome c
-
 Trypanosoma cruzi
1.6.2.4 FMN
-
 Trypanosoma cruzi
1.6.2.4 NADPH
-
 Trypanosoma cruzi