EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.31 | L367E | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.31 | L367F | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, in silico mutagenesis and structural modeling, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.31 | L367K | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.31 | L367W | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.33 | L367E | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.33 | L367F | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, in silico mutagenesis and structural modeling, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.33 | L367K | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
1.13.11.33 | L367W | site-directed mutagenesis, site-directed mutagenesis, the mutant shows reduced activity with O2 compared to the wild-type enzyme, L367 is involved in oxygen access, overview | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.31 | additional information | - |
additional information | free-energy distribution for oxygen inside the substrate-free rabbit 12/15-LOX, containing four nested free-energy isosurfaces with different energy levels, overview | Oryctolagus cuniculus | |
1.13.11.31 | 0.0052 | - |
O2 | pH 7.4, wild-type enzyme, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 0.007 | - |
O2 | pH 7.4, mutant L367W, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 0.009 | - |
O2 | pH 7.4, mutant L367E, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 0.009 | - |
O2 | pH 7.4, mutant L367K, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 0.0401 | - |
O2 | pH 7.4, mutant L367F, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | additional information | - |
additional information | free-energy distribution for oxygen inside the substrate-free rabbit 12/15-LOX, containing four nested free-energy isosurfaces with different energy levels, overview | Oryctolagus cuniculus | |
1.13.11.33 | 0.0052 | - |
O2 | pH 7.4, wild-type enzyme, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 0.007 | - |
O2 | pH 7.4, mutant L367W, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 0.009 | - |
O2 | pH 7.4, mutant L367E, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 0.009 | - |
O2 | pH 7.4, mutant L367K, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 0.0401 | - |
O2 | pH 7.4, mutant L367F, with substrate linoleic acid | Oryctolagus cuniculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | arachidonate + O2 | Oryctolagus cuniculus | - |
(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? | |
1.13.11.33 | arachidonate + O2 | Oryctolagus cuniculus | - |
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.31 | Oryctolagus cuniculus | - |
- |
- |
1.13.11.33 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | arachidonate + O2 | - |
Oryctolagus cuniculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? | |
1.13.11.31 | arachidonate + O2 | high oxygen affinity is important for effective catalysis, L367 is involved in oxygen access, channel structure, overview, arachidonic acid closes the substrate-binding pocket for oxygen diffusion but opens a fourth oxygen access channel | Oryctolagus cuniculus | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate | - |
? | |
1.13.11.31 | linoleic acid + O2 | reaction of EC 1.13.11.12 | Oryctolagus cuniculus | ? | - |
? | |
1.13.11.31 | additional information | the bifunctional enzyme also forms (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate, the product of 15-LO activity, EC 1.13.11.33, in a ratio of 9:1 15(S)-HPETE to 12(S)-HPETE | Oryctolagus cuniculus | ? | - |
? | |
1.13.11.33 | arachidonate + O2 | - |
Oryctolagus cuniculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.33 | arachidonate + O2 | high oxygen affinity is important for effective catalysis, L367 is involved in oxygen access, channel structure, overview, arachidonic acid closes the substrate-binding pocket for oxygen diffusion but opens a fourth oxygen access channel | Oryctolagus cuniculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? | |
1.13.11.33 | linoleic acid + O2 | reaction of EC 1.13.11.12 | Oryctolagus cuniculus | ? | - |
? | |
1.13.11.33 | additional information | the bifunctional enzyme also forms (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate, the product of 12-LO activity, EC 1.13.11.31, in a ratio of 9:1 15(S)-HPETE to 12(S)HPETE | Oryctolagus cuniculus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.31 | More | structural modeling, overview | Oryctolagus cuniculus |
1.13.11.33 | More | structural modeling, overview | Oryctolagus cuniculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.31 | 12/15-lipoxygenase | - |
Oryctolagus cuniculus |
1.13.11.31 | 12/15-LOX | - |
Oryctolagus cuniculus |
1.13.11.33 | 12/15-lipoxygenase | - |
Oryctolagus cuniculus |
1.13.11.33 | 12/15-LOX | - |
Oryctolagus cuniculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.31 | 0.3 | - |
O2 | pH 7.4, mutant L367K, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 2.2 | - |
O2 | pH 7.4, mutant L367E, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 4.4 | - |
O2 | pH 7.4, mutant L367W, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 5.6 | - |
O2 | pH 7.4, mutant L367F, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.31 | 13.7 | - |
O2 | pH 7.4, wild-type enzyme, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 0.3 | - |
O2 | pH 7.4, mutant L367K, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 2.2 | - |
O2 | pH 7.4, mutant L367E, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 4.4 | - |
O2 | pH 7.4, mutant L367W, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 5.6 | - |
O2 | pH 7.4, mutant L367F, with substrate linoleic acid | Oryctolagus cuniculus | |
1.13.11.33 | 13.7 | - |
O2 | pH 7.4, wild-type enzyme, with substrate linoleic acid | Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.31 | 7.4 | - |
assay at | Oryctolagus cuniculus |
1.13.11.33 | 7.4 | - |
assay at | Oryctolagus cuniculus |