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Literature summary extracted from

  • Chen, J.; Li, W.; Wang, M.; Zhu, G.; Liu, D.; Sun, F.; Hao, N.; Li, X.; Rao, Z.; Zhang, X.C.
    Crystal structure and mutagenic analysis of GDOsp, a gentisate 1,2-dioxygenase from Silicibacter pomeroyi (2008), Protein Sci., 17, 1362-1373.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.4 crystal structure at 2.8 A resolution, hanging drop vapor diffusion crystallization at 16°C. The crystal form belongs to the R32 space group, with two protein molecules (named as A and B) per asymmetric unit and 45% solvent content Ruegeria pomeroyi

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.4 D175N inactive mutant enzyme Ruegeria pomeroyi
1.13.11.4 D225N activity is comparable to wild-type activity Ruegeria pomeroyi
1.13.11.4 E47Q activity is comparable to wild-type activity Ruegeria pomeroyi
1.13.11.4 H119A/H121A despite its wild-type like structural propertie, the mutant shows extremely low gentisate 1,2-dioxygenase activity Ruegeria pomeroyi
1.13.11.4 H290A/H292A mutant can not be expressed in a soluble form Ruegeria pomeroyi
1.13.11.4 L39T activity is comparable to wild-type activity Ruegeria pomeroyi
1.13.11.4 P253_Y254del mutation reduces the activity to 30% Ruegeria pomeroyi
1.13.11.4 Q108E inactive mutant enzyme Ruegeria pomeroyi
1.13.11.4 Q108E/D175N activity is near zero Ruegeria pomeroyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.012
-
 2,5-Dihydroxybenzoate
-
 Ruegeria pomeroyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.4 Fe2+ two ferrous centers are located in its two homologous cupin domains, respectively. The N-terminal Fe2+-binding site is essential for the enzyme activity but dispensable for the protein overall tertiary structure Ruegeria pomeroyi

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Ruegeria pomeroyi Q5LLB1 DSS-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4
-
 Ruegeria pomeroyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Ruegeria pomeroyi maleylpyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.4 GDOsp
-
 Ruegeria pomeroyi
1.13.11.4 gtdA-2
-
 Ruegeria pomeroyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.4 70
-
 protein unfolds and loses its secondary structures above Ruegeria pomeroyi