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Literature summary extracted from

  • Woo, J.; Howell, M.H.; von Arnim, A.G.
    Structure-function studies on the active site of the coelenterazine-dependent luciferase from Renilla (2008), Protein Sci., 17, 725-735.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.12.5 molecular docking simulations with the coelenterazine substrate. Two triads of residues are critical for catalysis. Putative catalytic triad residues D120, E144, and H285 bear only limited resemblance to those of aequorin. Residues N53, W121, and P220 are also involved in catalysis Renilla reniformis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 A164W 73% of wild-type activity Renilla reniformis
1.13.12.5 D120E 1.1% of wild-type activity Renilla reniformis
1.13.12.5 D120F no activity detected Renilla reniformis
1.13.12.5 D120Y no activity detected Renilla reniformis
1.13.12.5 E144D 5.6% of wild-type activity Renilla reniformis
1.13.12.5 E144F no activity detected Renilla reniformis
1.13.12.5 E144Y no activity detected Renilla reniformis
1.13.12.5 E160N 27.2% of wild-type activity Renilla reniformis
1.13.12.5 F180C 14.3% of wild-type activity Renilla reniformis
1.13.12.5 F180T 5.4% of wild-type activity Renilla reniformis
1.13.12.5 F180Y 11.0% of wild-type activity Renilla reniformis
1.13.12.5 F180Y 61.8% of wild-type activity Renilla reniformis
1.13.12.5 F261A no activity detected Renilla reniformis
1.13.12.5 F261S no activity detected Renilla reniformis
1.13.12.5 H285A 11.3% of wild-type activity Renilla reniformis
1.13.12.5 H285D no activity detected Renilla reniformis
1.13.12.5 H285K no activity detected Renilla reniformis
1.13.12.5 H285N 0.1% of wild-type activity Renilla reniformis
1.13.12.5 I140L 113% of wild-type activity Renilla reniformis
1.13.12.5 I163F 11.0% of wild-type activity Renilla reniformis
1.13.12.5 I223W 0.2% of wild-type activity Renilla reniformis
1.13.12.5 K189D 24.7% of wild-type activity Renilla reniformis
1.13.12.5 K193S 54.8% of wild-type activity Renilla reniformis
1.13.12.5 K308I 47.5% of wild-type activity Renilla reniformis
1.13.12.5 M185G 16.7% of wild-type activity Renilla reniformis
1.13.12.5 N53C 3.4% of wild-type activity Renilla reniformis
1.13.12.5 N53G 0.5% of wild-type activity Renilla reniformis
1.13.12.5 N53H 2.1% of wild-type activity Renilla reniformis
1.13.12.5 N53M 1.8% of wild-type activity Renilla reniformis
1.13.12.5 N53P no activity detected Renilla reniformis
1.13.12.5 N53Q 25.1% of wild-type activity Renilla reniformis
1.13.12.5 N53R 90% of wild-type activity Renilla reniformis
1.13.12.5 N53S 20.7% of wild-type activity Renilla reniformis
1.13.12.5 P157R 101% of wild-type activity Renilla reniformis
1.13.12.5 P220C 72.7% of wild-type activity Renilla reniformis
1.13.12.5 P220E 4.9% of wild-type activity Renilla reniformis
1.13.12.5 P220F 15.7% of wild-type activity Renilla reniformis
1.13.12.5 P220G 548% of wild-type activity Renilla reniformis
1.13.12.5 P220L 500% of wild-type activity Renilla reniformis
1.13.12.5 P220M 140% of wild-type activity Renilla reniformis
1.13.12.5 P220Q 222% of wild-type activity Renilla reniformis
1.13.12.5 P220S 55.4% of wild-type activity Renilla reniformis
1.13.12.5 P220T 89.6% of wild-type activity Renilla reniformis
1.13.12.5 P220V 70.5% of wild-type activity Renilla reniformis
1.13.12.5 T184C 62.7% of wild-type activity Renilla reniformis
1.13.12.5 T184F 46.1% of wild-type activity Renilla reniformis
1.13.12.5 W121A 26.8% of wild-type activity Renilla reniformis
1.13.12.5 W121G 4.9% of wild-type activity Renilla reniformis
1.13.12.5 W121R 1.1% of wild-type activity Renilla reniformis
1.13.12.5 W121S 17.3% of wild-type activity Renilla reniformis
1.13.12.5 W121Y 3.1% of wild-type activity Renilla reniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 diethyldicarbonate
-
 Renilla reniformis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Renilla reniformis P27652
-
-