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Literature summary extracted from

  • Wang, N.C.; Ko, T.P.; Lee, C.Y.
    Inactive S298R disassembles the dodecameric L-aspartate 4-decarboxylase into dimers (2008), Biochem. Biophys. Res. Commun., 374, 134-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.12 expressed in Escherichia coli BL21(DE3)pLysS cells Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.12 P257H the mutation results in inactivation of the enzyme, but does not affect the overall structure (0.08% of the wild type enzymatic activity) Pseudomonas sp.
4.1.1.12 S298R the mutation disassembles the dodecameric L-aspartate 4-decarboxylase into inactive dimers (0.1% of the wild type enzymatic activity) Pseudomonas sp.
4.1.1.12 Y207H the mutation results in inactivation of the enzyme, but does not affect the overall structure (0.08% of the wild type enzymatic activity) Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.12 Pseudomonas sp.
-
 strain ATCC 19121
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.12 HiTrap chelating column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.12 0.02
-
 mutant enzyme P257H, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.
4.1.1.12 0.02
-
 mutant enzyme S298R, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.
4.1.1.12 0.02
-
 mutant enzyme Y207H, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.
4.1.1.12 20.3
-
 wild type enzyme, in acetate buffer (pH 5.0) at 40°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.12 L-Asp
-
 Pseudomonas sp. L-Ala + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.12 dodecamer at pH 5.0, X-ray crystallography Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.1.12 Asd
-
 Pseudomonas sp.
4.1.1.12 L-aspartate 4-decarboxylase
-
 Pseudomonas sp.