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Literature summary extracted from

  • Hopper, D.J.; Kaderbhai, M.A.; Marriott, S.A.; Young, M.; Rogozinski, J.
    Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp. (2002), Biochem. J., 367, 483-489.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.2.2 expression in Escherichia coli Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.2.2 periplasm
-
 Pseudomonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.2.2 Ca2+ heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.2.2 72256
-
 1 * 72256, calculated from sequence Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.2.2 lupanine + pyrroloquinoline quinone + H2O Pseudomonas sp. lupanine is a quinolizidine alkaloid produced by plants of the genus Lupinus. It is metabolized by a Pseudomonas sp. with conversion into 17-hydroxylupanine as the first step. The enzyme involved is induced by growth of the organism on lupanine. cofactors. It catalyses the dehydrogenation of lupanine with the PQQ acting as electron acceptor and the electrons are transferred to the haem before being passed to an external acceptor. This acceptor has not yet been identified but the enzyme rapidly reduces horse heart cytochrome c. Addition of water to the dehydrogenated lupanine gives the hydroxylated product 17-hydroxylupanine + pyrroloquinoline quinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.2.2 Pseudomonas sp. Q934G0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.2.2 recombinant enzyme Pseudomonas sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.17.2.2 heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.2.2 culture condition:lupanine-grown cell
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.2.2 lupanine + pyrroloquinoline quinone + H2O
-
 Pseudomonas sp. 17-hydroxylupanine + pyrroloquinoline quinol
-
 ?
1.17.2.2 lupanine + pyrroloquinoline quinone + H2O lupanine is a quinolizidine alkaloid produced by plants of the genus Lupinus. It is metabolized by a Pseudomonas sp. with conversion into 17-hydroxylupanine as the first step. The enzyme involved is induced by growth of the organism on lupanine. cofactors. It catalyses the dehydrogenation of lupanine with the PQQ acting as electron acceptor and the electrons are transferred to the haem before being passed to an external acceptor. This acceptor has not yet been identified but the enzyme rapidly reduces horse heart cytochrome c. Addition of water to the dehydrogenated lupanine gives the hydroxylated product Pseudomonas sp. 17-hydroxylupanine + pyrroloquinoline quinol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.2.2 monomer 1 * 72256, calculated from sequence Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.2.2 heme
-
 Pseudomonas sp.
1.17.2.2 pyrroloquinoline quinone
-
 Pseudomonas sp.