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Literature summary extracted from

  • Dionne, I.; Bell, S.D.
    Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins (2005), Biochem. J., 387, 859-863.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.27 additional information no activation of UDG1 by proliferating cell nuclear antigen, PCNA Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 expression of GST-tagged UDG1 in Escherichia coli, expression of UDG1 with PCNA in the yeast two-hybrid system using Saccharomyces cerevisiae strain AH109 Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.27 Alba protein a small basic chromatin protein, repression of UDG1 activity Saccharolobus solfataricus
3.2.2.27 Sso7d protein a small basic chromatin protein, slight repression of UDG1 activity Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Saccharolobus solfataricus the formation of archaeal chromatin is highly repressive to UDG1 activity, mechanistic basis for coupling UDG1 to the replication fork, modelling, overview uracil + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Saccharolobus solfataricus
-
 gene udg1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.27 additional information optimal growth at 80°C Saccharolobus solfataricus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O the formation of archaeal chromatin is highly repressive to UDG1 activity, mechanistic basis for coupling UDG1 to the replication fork, modelling, overview Saccharolobus solfataricus uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O UDG1 shows a marked preference for substrates containing a G-U base pair over either A-U or single-stranded uracil-containing DNA substrates, and it interacts with a single subunit of the heterotrimeric sliding clamp proliferating cell nuclear antigen, PCNA, in a PIP motif-dependent manner, a conserved C-terminal consensus interaction motif, overview Saccharolobus solfataricus uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 More the enzyme belongs to the archaeal family 4 Saccharolobus solfataricus
3.2.2.27 UDG1
-
 Saccharolobus solfataricus
3.2.2.27 uracil DNA glycosylase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 65
-
 recombinant enzyme Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.27 65 99
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 8
-
 assay at Saccharolobus solfataricus