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Literature summary extracted from

  • Zhuang, Z.; Song, F.; Zhao, H.; Li, L.; Cao, J.; Eisenstein, E.; Herzberg, O.; Dunaway-Mariano, D.
    Divergence of function in the hot dog fold enzyme superfamily: the bacterial thioesterase YciA (2008), Biochemistry, 47, 2789-2796.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 the gene encoding YciA is amplified by PCR and expressed in Escherichia coli strain BL21 (DE3) Escherichia coli
3.1.2.20 the gene encoding YciA is amplified by PCR and expressed in Escherichia coli strain BL21 (DE3) Haemophilus influenzae

General Stability

EC Number General Stability Organism
3.1.2.20 YciA is a highly soluble, robust protein Haemophilus influenzae
3.1.2.20 YciA tends to precipitate at high concentrations Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.20 4-hydroxyphenacyl-CoA
-
 Escherichia coli
3.1.2.20 4-hydroxyphenacyl-CoA
-
 Haemophilus influenzae
3.1.2.20 CoA strong feedback inhibitor, product inhibition Escherichia coli
3.1.2.20 CoA strong feedback inhibitor, product inhibition Haemophilus influenzae
3.1.2.20 hexyl-CoA
-
 Escherichia coli
3.1.2.20 hexyl-CoA
-
 Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 0.0016
-
 phenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0019
-
 n-octanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0021
-
 tiglyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0025
-
 myristoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0035
-
 n-butyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0037
-
 3-hydroxyphenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0041
-
 n-hexanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0042
-
 linoleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0045
-
 n-decanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0047
-
 acetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0059
-
 crotonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.006
-
 propionyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.006
-
 lauroyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0064
-
 palmitoleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0076
-
 4-hydroxyphenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.0081
-
 glutaryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.009
-
 beta-methylcrotonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.01
-
 stearoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.011
-
 oleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.011
-
 oleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.013
-
 isobutyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.013
-
 n-decanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.014
-
 isovaleryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.015
-
 isobutyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.016
-
 phenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.017
-
 palmitoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.02
-
 succinyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.02
-
 lauroyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.02
-
 DL-3-hydroxy-3-methylglutaryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.025
-
 4-hydroxybenzoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.031
-
 DL-3-hydroxybutyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.037
-
 acetoacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.039
-
 arachidoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.068
-
 n-butyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.14
-
 malonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.167
-
 acetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.17
-
 methylmalonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.29
-
 beta-methylcrotonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.8
-
 n-butyryl-pantetheine phosphate pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 1.4
-
 n-butyryl-pantetheine phosphate pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 33
-
 palmitoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.20 cytoplasm
-
 Escherichia coli 5737
-
3.1.2.20 cytoplasm
-
 Haemophilus influenzae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 15160
-
 His-tagged YciA protein, determined by electrospray ionization mass spectrometry Escherichia coli
3.1.2.20 16630
-
 His-tagged YciA protein, determined by electrospray ionization mass spectrometry, N-terminal methionine is removed Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.20 4-Hydroxybenzoyl-CoA + H2O Escherichia coli
-
 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.20 4-Hydroxybenzoyl-CoA + H2O Haemophilus influenzae
-
 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.20 acetyl-CoA + H2O Escherichia coli
-
 CoA + acetate
-
 ?
3.1.2.20 acetyl-CoA + H2O Haemophilus influenzae
-
 CoA + acetate
-
 ?
3.1.2.20 additional information Escherichia coli the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites ?
-
 ?
3.1.2.20 additional information Haemophilus influenzae the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites ?
-
 ?
3.1.2.20 additional information Haemophilus influenzae the substrate activity of propionyl-s-N-acetylcysteine is negligible and that of n-butyryl-pantetheine phosphate low, it is evident YciA does not target acylated holoacyl carrier proteins or other acylated proteins as substrates ?
-
 ?
3.1.2.20 additional information Escherichia coli YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis ?
-
 ?
3.1.2.20 additional information Haemophilus influenzae YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Escherichia coli P0A8Z0
-
-
3.1.2.20 Haemophilus influenzae P44886
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20 Cells are harvested by centrifugation and suspended in lysis buffer, passed through a French press and centrifuged. The supernatant is loaded onto a Ni-NTA agarose column. The column is washed with a buffer containing 50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole and 1 mM dithiothreitol, and then the YciA is eluted with an imidazole gradient up to 250 mM imidazole. The pooled and concentrated fractions are chromatographed on a size exclusion column using a buffer containing 50 mM NaH2PO4, 50 mM NaCl, 10 mM imidazole and 1 mM dithiothreitol. Escherichia coli
3.1.2.20 Cells are harvested by centrifugation, and the cell pellet is suspended in HEPES buffer containing 2 mM DTT and 0.2 mM phenylmethanesulfonyl fluoride. The cells are lysed in a French press and the lysate is centrifuged. The supernatant is loaded onto a DEAE Sepharose Fast Flow column equilibrated with HEPES buffer. A linear gradient of KCl in 50 mM Hepes is used to elute the protein. Desired fractions are pooled, concentrated and subjected to ammonium sulfate fractionation. The 40-60% ammonium sulfate fraction is centrifuged, the protein pellet is dissolved, loaded onto a size exclusion column and eluted with 10 mM HEPES containing 0.15 M KCl. Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 3-hydroxyphenylacetyl-CoA + H2O
-
 Haemophilus influenzae CoA + 3-hydroxyphenylacetate
-
 ?
3.1.2.20 4-Hydroxybenzoyl-CoA + H2O
-
 Escherichia coli 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.20 4-Hydroxybenzoyl-CoA + H2O
-
 Haemophilus influenzae 4-Hydroxybenzoate + CoA
-
 ?
3.1.2.20 4-hydroxyphenylacetyl-CoA + H2O
-
 Haemophilus influenzae CoA + 4-hydroxyphenylacetate
-
 ?
3.1.2.20 acetoacetyl-CoA + H2O
-
 Haemophilus influenzae CoA + acetoacetate
-
 ?
3.1.2.20 acetyl-CoA + H2O
-
 Escherichia coli CoA + acetate
-
 ?
3.1.2.20 acetyl-CoA + H2O
-
 Haemophilus influenzae CoA + acetate
-
 ?
3.1.2.20 arachidoyl-CoA + H2O
-
 Haemophilus influenzae CoA + arachidate
-
 ?
3.1.2.20 beta-methylcrotonyl-CoA + H2O
-
 Escherichia coli CoA + beta-methylcrotonate
-
 ?
3.1.2.20 beta-methylcrotonyl-CoA + H2O
-
 Haemophilus influenzae CoA + beta-methylcrotonate
-
 ?
3.1.2.20 crotonyl-CoA + H2O
-
 Haemophilus influenzae CoA + crotonate
-
 ?
3.1.2.20 DL-3-hydroxy-3-methylglutaryl-CoA + H2O
-
 Haemophilus influenzae CoA + 3-hydroxy-3-methylglutarate
-
 ?
3.1.2.20 DL-3-hydroxybutyryl-CoA + H2O
-
 Haemophilus influenzae CoA + ?
-
 ?
3.1.2.20 glutaryl-CoA + H2O
-
 Haemophilus influenzae CoA + glutarate
-
 ?
3.1.2.20 hexanoyl-CoA + H2O
-
 Haemophilus influenzae CoA + hexanoate
-
 ?
3.1.2.20 isobutyryl-CoA + H2O
-
 Escherichia coli CoA + isobutyrate
-
 ?
3.1.2.20 isobutyryl-CoA + H2O
-
 Haemophilus influenzae CoA + isobutyrate
-
 ?
3.1.2.20 isovaleryl-CoA + H2O
-
 Haemophilus influenzae CoA + isovalerate
-
 ?
3.1.2.20 lauroyl-CoA + H2O
-
 Escherichia coli CoA + laurate
-
 ?
3.1.2.20 lauroyl-CoA + H2O
-
 Haemophilus influenzae CoA + laurate
-
 ?
3.1.2.20 linoleoyl-CoA + H2O
-
 Haemophilus influenzae CoA + linolate
-
 ?
3.1.2.20 malonyl-CoA + H2O
-
 Haemophilus influenzae CoA + malonate
-
 ?
3.1.2.20 methylmalonyl-CoA + H2O
-
 Haemophilus influenzae CoA + methylmalonate
-
 ?
3.1.2.20 additional information the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites Escherichia coli ?
-
 ?
3.1.2.20 additional information the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites Haemophilus influenzae ?
-
 ?
3.1.2.20 additional information the substrate activity of propionyl-s-N-acetylcysteine is negligible and that of n-butyryl-pantetheine phosphate low, it is evident YciA does not target acylated holoacyl carrier proteins or other acylated proteins as substrates Haemophilus influenzae ?
-
 ?
3.1.2.20 additional information YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis Escherichia coli ?
-
 ?
3.1.2.20 additional information YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis Haemophilus influenzae ?
-
 ?
3.1.2.20 myristoyl-CoA + H2O
-
 Haemophilus influenzae CoA + myristate
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Escherichia coli CoA + n-butyrate
-
 ?
3.1.2.20 n-butyryl-CoA + H2O
-
 Haemophilus influenzae CoA + n-butyrate
-
 ?
3.1.2.20 n-butyryl-pantetheine phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.1.2.20 n-butyryl-pantetheine phosphate + H2O
-
 Haemophilus influenzae ?
-
 ?
3.1.2.20 n-decanoyl-CoA + H2O
-
 Escherichia coli CoA + n-decanoate
-
 ?
3.1.2.20 n-decanoyl-CoA + H2O
-
 Haemophilus influenzae CoA + n-decanoate
-
 ?
3.1.2.20 n-octanoyl-CoA + H2O
-
 Haemophilus influenzae CoA + n-octanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Escherichia coli CoA + oleate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Haemophilus influenzae CoA + oleate
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Haemophilus influenzae CoA + palmitoleoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Escherichia coli CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Haemophilus influenzae CoA + palmitate
-
 ?
3.1.2.20 phenylacetyl-CoA + H2O
-
 Escherichia coli CoA + phenylacetate
-
 ?
3.1.2.20 phenylacetyl-CoA + H2O
-
 Haemophilus influenzae CoA + phenylacetate
-
 ?
3.1.2.20 propionyl-CoA + H2O
-
 Haemophilus influenzae CoA + propionate
-
 ?
3.1.2.20 propionyl-s-N-acetylcysteamine + H2O
-
 Haemophilus influenzae CoA + ?
-
 ?
3.1.2.20 stearoyl-CoA + H2O
-
 Haemophilus influenzae CoA + stearate
-
 ?
3.1.2.20 succinyl-CoA + H2O
-
 Haemophilus influenzae CoA + succinate
-
 ?
3.1.2.20 tiglyl-CoA + H2O
-
 Haemophilus influenzae CoA + (2E)-2-methylbut-2-enoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 homohexamer determined by using equilibrium sedimentation techniques Haemophilus influenzae
3.1.2.20 homohexamer YciA determined by using equilibrium sedimentation techniques Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 acyl-CoA hydrolase
-
 Escherichia coli
3.1.2.20 acyl-CoA hydrolase
-
 Haemophilus influenzae
3.1.2.20 thioesterase
-
 Escherichia coli
3.1.2.20 thioesterase
-
 Haemophilus influenzae
3.1.2.20 YciA
-
 Escherichia coli
3.1.2.20 YciA
-
 Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.20 25
-
 assay at Escherichia coli
3.1.2.20 25
-
 assay at Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 additional information
-
 propionyl-S-n-acetylcysteamine absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured, Kcat is above 0.0001 Haemophilus influenzae
3.1.2.20 0.0062
-
 4-hydroxybenzoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.078
-
 4-hydroxybenzoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.082
-
 n-butyryl-pantetheine phosphate pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.1
-
 beta-methylcrotonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.27
-
 arachidoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.54
-
 beta-methylcrotonyl-CoA absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured Haemophilus influenzae
3.1.2.20 0.55
-
 acetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 0.77
-
 stearoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.8
-
 n-butyrylpantethiene phosphate pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 0.92
-
 crotonyl-CoA absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured Haemophilus influenzae
3.1.2.20 1.1
-
 methylmalonyl-CoA absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured Haemophilus influenzae
3.1.2.20 1.3
-
 acetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 1.7
-
 oleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 2
-
 linoleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 2.6
-
 tiglyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 2.7
-
 palmitoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 3.3
-
 malonyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 3.7
-
 palmitoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 4.6
-
 n-octanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 5.7
-
 palmitoleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 6.7
-
 n-butyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 6.9
-
 myristoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 8.3
-
 DL-3-hydroxybutyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 8.9
-
 DL-3-hydroxy-3-methylglutaryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 9.2
-
 lauroyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 9.4
-
 phenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 9.5
-
 isobutyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 9.6
-
 lauroyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 13
-
 oleoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 13
-
 n-decanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 15
-
 hexanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 17
-
 n-decanoyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Escherichia coli
3.1.2.20 22
-
 n-butyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 25
-
 propionyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 30
-
 isovaleryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 35
-
 acetoacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 75
-
 4-hydroxyphenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 100
-
 glutaryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 130
-
 phenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 140
-
 isobutyryl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 140
-
 3-hydroxyphenylacetyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae
3.1.2.20 190
-
 succinyl-CoA pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.20 7.5
-
 assay at Escherichia coli
3.1.2.20 7.5
-
 assay at Haemophilus influenzae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.20 additional information
-
 additional information noncompetitive inhibition of YciA-catalyzed isobutyryl-CoA hydrolysis is observed which defined a Kii of 0.0047 mM (Vmax altered) and a Kis of 0.33 microM (Vmax/Km altered) Haemophilus influenzae
3.1.2.20 0.0019
-
 hexyl-CoA
-
 Haemophilus influenzae
3.1.2.20 0.0054
-
 hexyl-CoA
-
 Escherichia coli
3.1.2.20 0.0058
-
 4-hydroxyphenacyl-CoA
-
 Haemophilus influenzae