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Literature summary extracted from
- Mutational analysis of the active site flap (20s loop) of mandelate racemase (2008), Biochemistry, 47, 566-578.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.2.2 | Recombinant MR from is overproduced from Escherichia coli strain BL21(DE3) cells transformed with a pET-15b plasmid containing the MR open reading frame. This construct encodes the MR gene product with an N-terminal hexahistidine tag. Mutants are produced by site-directed mutagenesis | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.1.2.2 | A25V | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | A25V | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | T24S | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | T24S | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V22A | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V22A | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V22F | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V22F | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V22I | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V22I | variation of the hydrophobic loop. For mandelate, catalytic efficiency similar to wild-type. For substrate 2-naphthylglycolate, increase in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V22I/V29L | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V22I/V29L | variation of the hydrophobic loop, catalytic efficiency similar to wild-type | Pseudomonas putida |
| 5.1.2.2 | V26A | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V26A | variation of the hydrophobic loop, catalytic efficiency similar to wild-type | Pseudomonas putida |
| 5.1.2.2 | V26A/V29L | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V26A/V29L | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V26F | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V26F | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V26L | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V26L | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V29A | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V29A | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V29F | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V29F | variation of the hydrophobic loop, decrease in catalytic efficiency | Pseudomonas putida |
| 5.1.2.2 | V29L | Catalytic efficiencies (kcat/Km) for all mutants are reduced between 6- and 40fold with the exception of V22I, V26A, V29L, and V22I/V29L which have near wildtype efficiencies with mandelate | Pseudomonas putida |
| 5.1.2.2 | V29L | variation of the hydrophobic loop. For mandelate, catalytic efficiency similar to wild-type. For substrate 2-naphthylglycolate, increase in catalytic efficiency | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.2.2 | 2-naphthohydroxamate | competitive inhibition at pH 7.5 | Pseudomonas putida |  |
| 5.1.2.2 | 2-naphtholhydroxamate | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.2.2 | 0.04 | - |
Mg2+ | pH 7.5, mutant V22A, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.04 | - |
Mg2+ | pH 7.5, mutant V22F, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.05 | - |
Mg2+ | pH 7.5, mutant V26F, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.08 | - |
Mg2+ | pH 7.5, mutant V29A, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.12 | - |
Mg2+ | pH 7.5, mutant V26L, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.15 | - |
Mg2+ | pH 7.5, mutant V26A, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.22 | - |
Mg2+ | pH 7.5, mutant V29F, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.29 | - |
Mg2+ | pH 7.5, mutant T24S, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.34 | - |
Mg2+ | pH 7.5, wild-type, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.47 | - |
Mg2+ | pH 7.5, mutant V29L, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.51 | - |
Mg2+ | pH 7.5, mutant V26A/V29L, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.55 | - |
(S)-2-naphthylglycolate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.55 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 0.6 | - |
(S)-Mandelate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.6 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 0.62 | - |
(S)-2-naphthylglycolate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.62 | - |
(S)-2-naphthylglycolate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.62 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 0.62 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 0.64 | - |
Mg2+ | pH 7.5, mutant A25V, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 0.74 | - |
(S)-2-naphthylglycolate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.74 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 0.8 | - |
(S)-2-naphthylglycolate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.8 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 0.9 | - |
(R)-mandelate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.9 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 0.91 | - |
(R)-mandelate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.91 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 0.93 | - |
(S)-2-naphthylglycolate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.93 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 0.97 | - |
(S)-Mandelate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.97 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1 | - |
(S)-2-naphthylglycolate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1.07 | - |
(S)-Mandelate | pH 7.5 at 25°C, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1.07 | - |
(S)-Mandelate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(R)-mandelate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-2-naphthylglycolate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(R)-2-naphthylglycolate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 1.1 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 1.2 | - |
(R)-mandelate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.2 | - |
(R)-2-naphthylglycolate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.2 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1.2 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1.3 | - |
(R)-2-naphthylglycolate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.3 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 1.4 | - |
(S)-2-naphthylglycolate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.4 | - |
(R)-mandelate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.4 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 1.4 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 1.47 | - |
(R)-mandelate | pH 7.5 at 25°C, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1.47 | - |
(R)-mandelate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1.5 | - |
(S)-Mandelate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1.5 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 1.55 | - |
(S)-2-naphthylglycolate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.55 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 1.6 | - |
(R)-2-naphthylglycolate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.6 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 1.6 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 1.6 | - |
(S)-2-naphthylglycolate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1.67 | - |
(S)-2-naphthylglycolate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.67 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(S)-Mandelate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(S)-Mandelate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(S)-2-naphthylglycolate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(R)-2-naphthylglycolate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(R)-mandelate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(R)-mandelate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 1.7 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 1.8 | - |
(S)-Mandelate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.8 | - |
(R)-mandelate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.8 | - |
(R)-2-naphthylglycolate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.8 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 1.8 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 1.8 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 1.9 | - |
(R)-2-naphthylglycolate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.9 | - |
(R)-2-naphthylglycolate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 1.9 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 1.9 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 2.1 | - |
(R)-2-naphthylglycolate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 2.1 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 2.2 | - |
(R)-2-naphthylglycolate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 2.2 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 2.4 | - |
(S)-2-naphthylglycolate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 2.4 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 2.8 | - |
(R)-mandelate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 2.8 | - |
(R)-mandelate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 2.8 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 2.8 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I/V29L | Pseudomonas putida | |
| 5.1.2.2 | 2.9 | - |
(R)-mandelate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 2.9 | - |
(S)-2-naphthylglycolate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 2.9 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 2.9 | - |
(S)-2-naphthylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 3 | - |
(S)-Mandelate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 3 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 3.2 | - |
(R)-2-naphthylglycolate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 3.2 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 3.4 | - |
(S)-Mandelate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 3.4 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 3.5 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 3.5 | - |
(R)-2-naphthylglycolate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 3.7 | - |
(R)-2-naphthylglycolate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 3.7 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I/V29L | Pseudomonas putida | |
| 5.1.2.2 | 3.8 | - |
Mg2+ | pH 7.5, mutant V22I, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 3.9 | - |
(S)-Mandelate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 3.9 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 4.2 | - |
(R)-2-naphthylglycolate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 4.2 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 4.2 | - |
Mg2+ | pH 7.5, mutant V22I/V29L, addition of either 10 mM (R)- or (S)-mandelate | Pseudomonas putida | |
| 5.1.2.2 | 4.4 | - |
(R)-mandelate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 4.4 | - |
(R)-mandelate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 4.4 | - |
(R)-2-naphthylglycolate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 4.4 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 4.4 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 4.4 | - |
(R)-2-naphtylglycolate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 5 | - |
(R)-mandelate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 5 | - |
(R)-mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 6.1 | - |
(S)-Mandelate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 6.1 | - |
(S)-Mandelate | pH 7.5 at 25°C, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.2.2 | Mg2+ | catalysis is Mg2+-dependent | Pseudomonas putida | |
| 5.1.2.2 | Mg2+ | Km value for wild-type, 0.34 mM | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.2.2 | 40700 | - |
mutants V22A, V26A and V29A | Pseudomonas putida |
| 5.1.2.2 | 40710 | - |
mutants V26A/V26L and T24S | Pseudomonas putida |
| 5.1.2.2 | 40730 | - |
wild type | Pseudomonas putida |
| 5.1.2.2 | 40740 | - |
mutants V22I, V26L and V29L | Pseudomonas putida |
| 5.1.2.2 | 40760 | - |
mutants A25V and V22I/V29L | Pseudomonas putida |
| 5.1.2.2 | 40780 | - |
mutants V22F, V26F and V29F | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.2.2 | (S)-mandelate | Pseudomonas putida | catalyzes the Mg2+-dependent 1,1-proton transfer that interconverts the enantiomers, catalysis is done via a two-base mechanism | (R)-mandelate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.2.2 | Pseudomonas putida | P11444 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.2.2 | by metal ion affinity chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.2.2 | (R)-2-naphthylglycolate | - |
Pseudomonas putida | (S)-2-naphthylglycolate | - |
r | |
| 5.1.2.2 | (R)-2-naphtylglycolate | - |
Pseudomonas putida | (S)-2-naphthylglycolate | - |
r | |
| 5.1.2.2 | (R)-mandelate | - |
Pseudomonas putida | (S)-mandelate | - |
r | |
| 5.1.2.2 | (S)-2-naphthylglycolate | - |
Pseudomonas putida | (R)-2-naphthylglycolate | - |
r | |
| 5.1.2.2 | (S)-2-naphthylglycolate | racemisation | Pseudomonas putida | (R)-2-naphthylglycolate | - |
r | |
| 5.1.2.2 | (S)-mandelate | - |
Pseudomonas putida | (R)-mandelate | - |
r | |
| 5.1.2.2 | (S)-mandelate | catalyzes the Mg2+-dependent 1,1-proton transfer that interconverts the enantiomers, catalysis is done via a two-base mechanism | Pseudomonas putida | (R)-mandelate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.2.2 | mandelate racemase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.2.2 | 25 | - |
assay at | Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.2.2 | 2.95 | - |
(S)-2-naphthylglycolate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 2.95 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 3 | 6 | (R)-mandelate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 3 | 6 | (R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 5.4 | - |
(R)-2-naphthylglycolate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 5.4 | - |
(S)-2-naphthylglycolate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 5.4 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 5.4 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 5.8 | - |
(S)-2-naphthylglycolate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 5.8 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 7.1 | - |
(S)-2-naphthylglycolate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 7.1 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 7.3 | - |
(R)-2-naphthylglycolate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 7.3 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 8.2 | - |
(S)-2-naphthylglycolate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 8.2 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 8.9 | - |
(S)-2-naphthylglycolate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 8.9 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 9.7 | - |
(S)-2-naphthylglycolate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 9.7 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 9.8 | - |
(S)-2-naphthylglycolate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 9.8 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 10.4 | - |
(R)-2-naphthylglycolate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 10.4 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 10.5 | - |
(R)-2-naphthylglycolate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 10.5 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22I/V29L | Pseudomonas putida | |
| 5.1.2.2 | 11.1 | - |
(S)-2-naphthylglycolate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 11.1 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 13 | - |
(R)-mandelate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 13 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 14 | - |
(S)-2-naphthylglycolate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 14 | - |
(R)-2-naphthylglycolate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 14 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 14 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 14.1 | - |
(R)-2-naphthylglycolate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 14.1 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 15.6 | - |
(R)-2-naphthylglycolate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 15.6 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 18.3 | - |
(R)-2-naphthylglycolate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 18.3 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 22 | - |
(S)-Mandelate | mutant A25V, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 22 | - |
(S)-Mandelate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 22 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant A25V | Pseudomonas putida | |
| 5.1.2.2 | 22 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 23 | - |
(R)-2-naphthylglycolate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 23 | - |
(S)-Mandelate | mutant V26L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 23 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 23 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26L | Pseudomonas putida | |
| 5.1.2.2 | 30 | - |
(R)-2-naphthylglycolate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 30 | - |
(S)-2-naphthylglycolate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 30 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 30 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 32 | - |
(S)-2-naphthylglycolate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 32 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 33 | - |
(R)-mandelate | mutant V26F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 33 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26F | Pseudomonas putida | |
| 5.1.2.2 | 38.4 | - |
(S)-2-naphthylglycolate | pH 7.5, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 38.4 | - |
(S)-2-naphthylglycolate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 46 | - |
(R)-2-naphthylglycolate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 46 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 53 | - |
(R)-2-naphthylglycolate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 53 | - |
(S)-Mandelate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 53 | - |
(R)-mandelate | mutant V29F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 53 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 53 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 53 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V29F | Pseudomonas putida | |
| 5.1.2.2 | 65 | - |
(S)-2-naphthylglycolate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 65 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 70 | - |
(R)-2-naphtylglycolate | pH 7.5, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 70 | - |
(R)-2-naphthylglycolate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 77 | - |
(S)-Mandelate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 77 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+ mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 82 | - |
(R)-mandelate | mutant V22A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 82 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V22A | Pseudomonas putida | |
| 5.1.2.2 | 89 | - |
(S)-Mandelate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 89 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 98 | - |
(S)-Mandelate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 98 | - |
(R)-mandelate | mutant V22F, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 98 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 98 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V22F | Pseudomonas putida | |
| 5.1.2.2 | 101 | - |
(S)-2-naphthylglycolate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 101 | - |
(S)-2-naphthylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 102 | - |
(R)-mandelate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 102 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 106 | - |
(R)-mandelate | mutant V26A/V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 106 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 120 | - |
(S)-Mandelate | mutant T24S, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 120 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant T24S | Pseudomonas putida | |
| 5.1.2.2 | 137 | - |
(R)-2-naphthylglycolate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 137 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 147 | - |
(R)-2-naphthylglycolate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 147 | - |
(R)-2-naphtylglycolate | pH 7.5, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 176 | - |
(S)-Mandelate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 176 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 180 | - |
(S)-Mandelate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 180 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 240 | - |
(R)-mandelate | mutant V29A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 240 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V29A | Pseudomonas putida | |
| 5.1.2.2 | 300 | - |
(R)-mandelate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 300 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V29L | Pseudomonas putida | |
| 5.1.2.2 | 304 | - |
(R)-mandelate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 304 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 470 | - |
(S)-Mandelate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 470 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 492 | - |
(S)-Mandelate | pH 7.5, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 492 | - |
(S)-Mandelate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 550 | - |
(S)-Mandelate | mutant V26A, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 550 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26A | Pseudomonas putida | |
| 5.1.2.2 | 552 | - |
(R)-mandelate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 552 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 620 | - |
(R)-mandelate | pH 7.5, 20 mM Mg2+, wild-type | Pseudomonas putida | |
| 5.1.2.2 | 620 | - |
(R)-mandelate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 710 | - |
(S)-Mandelate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 710 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V26A/V29L | Pseudomonas putida | |
| 5.1.2.2 | 740 | - |
(S)-Mandelate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 740 | - |
(S)-Mandelate | pH 7.5, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
| 5.1.2.2 | 1010 | - |
(R)-mandelate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1010 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V22I/V29L | Pseudomonas putida | |
| 5.1.2.2 | 1150 | - |
(R)-mandelate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 1150 | - |
(R)-mandelate | pH 7.5, 3.3 mM Mg2+, mutant V22I | Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.2.2 | 7.5 | - |
assay at | Pseudomonas putida |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.2.2 | 0.023 | - |
2-naphthohydroxamate | wild-type, 20 mM Mg2+, pH 7.5 | Pseudomonas putida | |
| 5.1.2.2 | 0.023 | - |
2-naphtholhydroxamate | wild-type, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 0.027 | - |
2-naphtholhydroxamate | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.027 | - |
2-naphthohydroxamate | wild-type, 3.3 mM Mg2+, pH 7.5 | Pseudomonas putida | |
| 5.1.2.2 | 0.035 | - |
2-naphthohydroxamate | mutant V29L, 3.3 mM Mg2+, pH 7.5 | Pseudomonas putida | |
| 5.1.2.2 | 0.035 | - |
2-naphtholhydroxamate | mutant V29L, pH 7.5, 25°C | Pseudomonas putida | |
| 5.1.2.2 | 0.112 | - |
2-naphtholhydroxamate | mutant V22I/V29L, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
| 5.1.2.2 | 0.112 | - |
2-naphthohydroxamate | mutant V29I/V29I, 20 mM Mg2+, pH 7.5 | Pseudomonas putida | |
| 5.1.2.2 | 0.19 | - |
2-naphthohydroxamate | mutant V22I, 3.3 mM Mg2+, pH 7.5 | Pseudomonas putida | |
| 5.1.2.2 | 0.19 | - |
2-naphtholhydroxamate | mutant V22I, pH 7.5, 25°C, presence of 20 mM Mg2+ | Pseudomonas putida | |
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Release 2023.1 (January 2023)
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