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Literature summary extracted from

  • Chakraborty, S.; Nemeria, N.S.; Balakrishnan, A.; Brandt, G.S.; Kneen, M.M.; Yep, A.; McLeish, M.J.; Kenyon, G.L.; Petsko, G.A.; Ringe, D.; Jordan, F.
    Detection and time course of formation of major thiamin diphosphate-bound covalent intermediates derived from a chromophoric substrate analogue on benzoylformate decarboxylase (2009), Biochemistry, 48, 981-994.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli BL21(DE3)pLysS cells Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 in complex with (E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid or 3-(pyridin-3-yl)acrylaldehyde, hanging drop vapour diffusion method, in 100 mM TrisHCl (pH 8.5), 150 mM CaCl2, 0.5% (v/v) MPD [2-methyl-2,4-pentanediol], and 22% (v/v) PEG 400 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 (E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid
-
 Pseudomonas putida 3-(pyridin-3-yl)acrylaldehyde + CO2
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFDC
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate dependent on Pseudomonas putida