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Literature summary extracted from

  • Fan, K.; Wei, P.; Feng, Q.; Chen, S.; Huang, C.; Ma, L.; Lai, B.; Pei, J.; Liu, Y.; Chen, J.; Lai, L.J.
    Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase (2004), Biol. Chem., 279, 1637-1642.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.69 expression in Escherichia coli Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.69 0.286
-
TFTRLQSLENV pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.549
-
FYPKLQASQAW pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 0.583
-
SGVTFQGKFKK pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1.15
-
TSAVLQSGFRK-NH2 pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1.44
-
ATVRLQAGNAT pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1.94
-
PHTVLQAVGAC pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.69 Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.69
-
Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.69 ATVRLQAGNAT + H2O
-
Severe acute respiratory syndrome-related coronavirus ATVRLQ + AGNAT
-
?
3.4.22.69 FYPKLQASQAW + H2O
-
Severe acute respiratory syndrome-related coronavirus FYPKLQ + ASQAW
-
?
3.4.22.69 KVATVQSKMSD + H2O weak activity Severe acute respiratory syndrome-related coronavirus KVATVQ + SKMSD
-
?
3.4.22.69 additional information cuts the 11 peptides covering all of the 11 cleavage sites on the viral polyprotein with different efficiency Severe acute respiratory syndrome-related coronavirus ?
-
?
3.4.22.69 NRATLQAIASE + H2O weak activity Severe acute respiratory syndrome-related coronavirus NRATLQ + AIASE
-
?
3.4.22.69 NVATLQAENVT + H2O weak activity Severe acute respiratory syndrome-related coronavirus NVATLQ + AENVT
-
?
3.4.22.69 PHTVLQAVGAC + H2O
-
Severe acute respiratory syndrome-related coronavirus PHTVLQ + AVGAC
-
?
3.4.22.69 REPLMQSADAS + H2O weak activity Severe acute respiratory syndrome-related coronavirus REPLMQ + SADAS
-
?
3.4.22.69 SAVKLQNNELS + H2O weak activity Severe acute respiratory syndrome-related coronavirus SAVKLQ + NNELS
-
?
3.4.22.69 SGVTFQGKFKK + H2O highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones Severe acute respiratory syndrome-related coronavirus SGVTFQ + GKFKK
-
?
3.4.22.69 TFTRLQSLENV + H2O
-
Severe acute respiratory syndrome-related coronavirus TFTRLQ + SLENV
-
?
3.4.22.69 TSAVLQSGFRK-NH2 + H2O highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones Severe acute respiratory syndrome-related coronavirus TSAVLQ + SGFRK-NH2
-
?

Subunits

EC Number Subunits Comment Organism
3.4.22.69 dimer a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml. The dimer may be the biological functional form of the protein Severe acute respiratory syndrome-related coronavirus
3.4.22.69 monomer a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml. The dimer may be the biological functional form of the protein Severe acute respiratory syndrome-related coronavirus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.69 SARS 3C-like proteinase
-
Severe acute respiratory syndrome-related coronavirus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.69 61
-
Tm-value, sigmoid denaturation curve Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.69 0.847
-
TFTRLQSLENV pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1.57
-
FYPKLQASQAW pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 1.68
-
PHTVLQAVGAC pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 2.55
-
SGVTFQGKFKK pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 3.29
-
ATVRLQAGNAT pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus
3.4.22.69 12.2
-
TSAVLQSGFRK-NH2 pH 7.3, room temperature Severe acute respiratory syndrome-related coronavirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.69 7
-
substrate: TSAVLQSGFRK-NH2 Severe acute respiratory syndrome-related coronavirus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.69 6 9 pH 6: about 50% of maximal activity, pH 9: about 65% of maximal activity, substrate: TSAVLQSGFRK-NH2 Severe acute respiratory syndrome-related coronavirus