EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.69 | expression in Escherichia coli | Severe acute respiratory syndrome-related coronavirus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.69 | 0.286 | - |
TFTRLQSLENV | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 0.549 | - |
FYPKLQASQAW | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 0.583 | - |
SGVTFQGKFKK | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 1.15 | - |
TSAVLQSGFRK-NH2 | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 1.44 | - |
ATVRLQAGNAT | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 1.94 | - |
PHTVLQAVGAC | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.69 | Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.69 | - |
Severe acute respiratory syndrome-related coronavirus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.69 | ATVRLQAGNAT + H2O | - |
Severe acute respiratory syndrome-related coronavirus | ATVRLQ + AGNAT | - |
? | |
3.4.22.69 | FYPKLQASQAW + H2O | - |
Severe acute respiratory syndrome-related coronavirus | FYPKLQ + ASQAW | - |
? | |
3.4.22.69 | KVATVQSKMSD + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | KVATVQ + SKMSD | - |
? | |
3.4.22.69 | additional information | cuts the 11 peptides covering all of the 11 cleavage sites on the viral polyprotein with different efficiency | Severe acute respiratory syndrome-related coronavirus | ? | - |
? | |
3.4.22.69 | NRATLQAIASE + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | NRATLQ + AIASE | - |
? | |
3.4.22.69 | NVATLQAENVT + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | NVATLQ + AENVT | - |
? | |
3.4.22.69 | PHTVLQAVGAC + H2O | - |
Severe acute respiratory syndrome-related coronavirus | PHTVLQ + AVGAC | - |
? | |
3.4.22.69 | REPLMQSADAS + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | REPLMQ + SADAS | - |
? | |
3.4.22.69 | SAVKLQNNELS + H2O | weak activity | Severe acute respiratory syndrome-related coronavirus | SAVKLQ + NNELS | - |
? | |
3.4.22.69 | SGVTFQGKFKK + H2O | highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones | Severe acute respiratory syndrome-related coronavirus | SGVTFQ + GKFKK | - |
? | |
3.4.22.69 | TFTRLQSLENV + H2O | - |
Severe acute respiratory syndrome-related coronavirus | TFTRLQ + SLENV | - |
? | |
3.4.22.69 | TSAVLQSGFRK-NH2 + H2O | highest cleavage efficiency. The two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive ones | Severe acute respiratory syndrome-related coronavirus | TSAVLQ + SGFRK-NH2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.69 | dimer | a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml. The dimer may be the biological functional form of the protein | Severe acute respiratory syndrome-related coronavirus |
3.4.22.69 | monomer | a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml. The dimer may be the biological functional form of the protein | Severe acute respiratory syndrome-related coronavirus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.69 | SARS 3C-like proteinase | - |
Severe acute respiratory syndrome-related coronavirus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.69 | 61 | - |
Tm-value, sigmoid denaturation curve | Severe acute respiratory syndrome-related coronavirus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.69 | 0.847 | - |
TFTRLQSLENV | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 1.57 | - |
FYPKLQASQAW | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 1.68 | - |
PHTVLQAVGAC | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 2.55 | - |
SGVTFQGKFKK | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 3.29 | - |
ATVRLQAGNAT | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus | |
3.4.22.69 | 12.2 | - |
TSAVLQSGFRK-NH2 | pH 7.3, room temperature | Severe acute respiratory syndrome-related coronavirus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.69 | 7 | - |
substrate: TSAVLQSGFRK-NH2 | Severe acute respiratory syndrome-related coronavirus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.69 | 6 | 9 | pH 6: about 50% of maximal activity, pH 9: about 65% of maximal activity, substrate: TSAVLQSGFRK-NH2 | Severe acute respiratory syndrome-related coronavirus |