Any feedback?
Literature summary extracted from
- Characterisation of the tryptophan synthase alpha subunit in maize (2008), BMC Plant Biol., 8, 44.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.8 | into the vectorS pET28a, pEZS-NL and pPCVE35E | Zea mays |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.8 | 0.458 | - |
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.2.8 | plastid | - |
Zea mays | 9536 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.8 | (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | Zea mays | - |
indole + D-glyceraldehyde 3-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.8 | Zea mays | B2Y0K4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.8 | recombinant protein by His-tag affinity purification via Ni-NTA agarose, plant protein by anion exchange and gel permeation chromatography | Zea mays |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.2.8 | leaf | - |
Zea mays | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.8 | (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays | indole + D-glyceraldehyde 3-phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.8 | tryptophan synthase alpha subunit | - |
Zea mays |
| 4.1.2.8 | ZmTSA | - |
Zea mays |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.8 | 30 | - |
tryptophan synthase assay | Zea mays |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.8 | 0.006 | - |
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.8 | 8.2 | - |
tryptophan synthase assay | Zea mays |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
