EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.2 | hydroxocobalamin | supplementation of hydroxocobalamin results in a marked increase in the holo-MCM activity in a dose-dependent manner, although the holo-MCM activity does not exceed 30% of the total-MCM activity even if hydroxocobalamin is supplemented at 10 mM | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.2 | expressed in Escherichia coli BL21 (DE3) cells and COS-7 cells | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.2 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.2 | Ni-NTA agarose resin chromatography | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.4.99.2 | liver | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Rattus norvegicus | succinyl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.2 | L-methylmalonyl-co-enzyme-A mutase | - |
Rattus norvegicus |
5.4.99.2 | L-methylmalonyl-CoA mutase | - |
Rattus norvegicus |
5.4.99.2 | MCM | - |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.2 | adenosylcobalamin | dependent on adenosylcobalamin as prosthetic group, under cobalamin-deficient conditions, (R)-2-methylmalonyl-CoA and its precursor, propionyl-CoA, increase as a result of a decrease in the catalytic activity of MCM due to deficiency of adenosylcobalamin | Rattus norvegicus |