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Literature summary extracted from

  • Wang, S.L.; Chen, S.J.; Wang, C.L.
    Purification and characterization of chitinases and chitosanases from a new species strain Pseudomonas sp. TKU015 using shrimp shells as a substrate (2008), Carbohydr. Res., 343, 1171-1179.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.132 Triton X-100 2% nonionic surfactant of Triton X-100 activates by 51% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Tween 20 2% nonionic surfactant of Tween 20 activates by 38% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Tween 40 2% nonionic surfactant of Tween 40 activates by 6% at 25°C for 30 min Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Cu2+ inhibits CHT1 Pseudomonas sp.
3.2.1.14 Fe2+ complete inhibition of CHT1 Pseudomonas sp.
3.2.1.14 Mn2+ complete inhibition of CHT1 Pseudomonas sp.
3.2.1.14 SDS partial inhibition at 2 mM Pseudomonas sp.
3.2.1.14 Tween-20 21% inhibition at 2% Pseudomonas sp.
3.2.1.132 Cu2+ 5 mM completely inhibits at 25°C for 30 min Pseudomonas sp.
3.2.1.132 EDTA 10 mM inhibits by 86% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Fe2+ 5 mM inhibits by 67% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Mn2+ 5 mM completely inhibits at 25°C for 30 min Pseudomonas sp.
3.2.1.132 PMSF 5 mM completely inhibits at 25°C for 30 min Pseudomonas sp.
3.2.1.132 SDS 2 mM of the anionic surfactant SDS completely inhibits at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Triton X-100 0.5% nonionic surfactant of Triton X-100 inhibits by 56% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Tween 20 0.5% nonionic surfactant of Tween 20 inhibits by 16% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Tween 40 0.5% nonionic surfactant of Tween 40 inhibits by 29% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 Zn2+ 5 mM inhibits by 21% at 25°C for 30 min Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ 5 mM activates by 11% at 25°C for 30 min Pseudomonas sp.
3.2.1.132 additional information 5 mM of Mg2+, Ba2+ have no effect on activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 68000
-
 gel filtration Pseudomonas sp.
3.2.1.132 30000
-
 x * 30000, SDS–PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Pseudomonas sp.
-
-
-
3.2.1.14 Pseudomonas sp. TKU015
-
-
-
3.2.1.132 Pseudomonas sp.
-
-
-
3.2.1.132 Pseudomonas sp. TKU015
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme 6fold from strain TKU015 by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration Pseudomonas sp.
3.2.1.132 by gel filtration, to homogeneity, 6.4fold Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 additional information strain TKU015 grows with shrimp shell wastes as the sole carbon and nitrogen source Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 0.0069
-
 purified CHT1, substrate chitin Pseudomonas sp.
3.2.1.14 0.0229
-
 purified CHT1, substrate colloidal chitin Pseudomonas sp.
3.2.1.132 9.3
-
 culture supernatant Pseudomonas sp.
3.2.1.132 59.1
-
 6.4fold purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O CHT1 is specific for chitin substrates Pseudomonas sp. ?
-
 ?
3.2.1.14 chitin + H2O CHT1 is specific for chitin substrates Pseudomonas sp. TKU015 ?
-
 ?
3.2.1.14 colloidal chitin + H2O CHT1 is specific for chitin substrates Pseudomonas sp. ?
-
 ?
3.2.1.14 colloidal chitin + H2O CHT1 is specific for chitin substrates Pseudomonas sp. TKU015 ?
-
 ?
3.2.1.132 chitosan + H2O activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan Pseudomonas sp. ?
-
 ?
3.2.1.132 chitosan + H2O activity toward 94% deacetylated soluble chitosan, 85% deacetylated soluble chitosan, and 73% deacetylated soluble chitosan Pseudomonas sp. TKU015 ?
-
 ?
3.2.1.132 additional information no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan Pseudomonas sp. ?
-
 ?
3.2.1.132 additional information no activity toward 82% deacetylated soluble chitosan, 73% deacetylated soluble chitosan, colloidal chitin, carboxymethyl cellulose, and xylan Pseudomonas sp. TKU015 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 68000, CHT1, SDS-PAGE Pseudomonas sp.
3.2.1.132 ? x * 30000, SDS–PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 CHT1
-
 Pseudomonas sp.
3.2.1.132 Chs1
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
 CHT1 Pseudomonas sp.
3.2.1.132 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 stable below, purified CHT1 Pseudomonas sp.
3.2.1.132 25 40 maintains its initial activity, has almost half of its activity at 50°C but is completely inactivated at 70°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
 CHT1 Pseudomonas sp.
3.2.1.132 4
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 5 7 purified CHT1 Pseudomonas sp.
3.2.1.132 3 9
-
 Pseudomonas sp.