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Literature summary extracted from

  • Gocke, D.; Walter, L.; Gauchenova, E.; Kolter, G.; Knoll, M.; Berthold, C.L.; Schneider, G.; Pleiss, J.; Mueller, M.; Pohl, M.
    Rational protein design of ThDP-dependent enzymes-engineering stereoselectivity (2008), ChemBioChem, 9, 406-412.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 mutant enzyme L461A, hanging drop vapour diffusion method Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 A460G mutant exhibits a higher Km value compared to the wild type enzyme Pseudomonas putida
4.1.1.7 L461A the mutant catalyzes the S-stereoselective addition of larger acceptor aldehydes, such as propanal with benzaldehyde and its derivatives (a reaction not catalyzed by the wild type enzyme) Pseudomonas putida
4.1.1.7 L461G the mutant catalyzes the S-stereoselective addition of larger acceptor aldehydes, such as propanal with benzaldehyde and its derivatives (a reaction not catalyzed by the wild type enzyme) Pseudomonas putida
4.1.1.7 L461V mutant exhibits a higher Km value compared to the wild type enzyme Pseudomonas putida
4.1.1.7 P24A mutant exhibits a higher Km value compared to the wild type enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 0.06
-
 benzoylformate mutant enzyme L461V, in potassium phosphate buffer (pH 6.5, 50 mM) Pseudomonas putida
4.1.1.7 0.25
-
 benzoylformate mutant enzyme L461A, in potassium phosphate buffer (pH 6.5, 50 mM) Pseudomonas putida
4.1.1.7 0.37
-
 benzoylformate wild type enzyme, in potassium phosphate buffer (pH 6.5, 50 mM) Pseudomonas putida
4.1.1.7 0.51
-
 benzoylformate mutant enzyme P24A, in potassium phosphate buffer (pH 6.5, 50 mM) Pseudomonas putida
4.1.1.7 1.4
-
 benzoylformate mutant enzyme L461G, in potassium phosphate buffer (pH 6.5, 50 mM) Pseudomonas putida
4.1.1.7 1.54
-
 benzoylformate mutant enzyme A460G, in potassium phosphate buffer (pH 6.5, 50 mM) Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Ni-NTA column chromatography and Superdex G200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzaldehyde + acetaldehyde
-
 Pseudomonas putida (S)-2-hydroxy-1-phenylpropan-1-one
-
 ?
4.1.1.7 benzaldehyde + benzaldehyde
-
 Pseudomonas putida (R)-benzoin
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
4.1.1.7 additional information the S-stereoselective addition of larger acceptor aldehydes, such as propanal with benzaldehyde and its derivatives is not catalyzed by the wild type enzyme, but with mutant enzyme L461A Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
 Pseudomonas putida