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Literature summary extracted from

  • Rothacker, B.; Ilg, T.
    Functional characterization of a Drosophila melanogaster succinic semialdehyde dehydrogenase and a non-specific aldehyde dehydrogenase (2008), Insect Biochem. Mol. Biol., 38, 354-366.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.24 PCR product cloned into pCR2.1-Topo. Open reading frame ligated into vector QE30. Overexpressed in Escherichia coli M15. Subcloned into pMALc2x and expressed in Escherichia coli TB1 Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.24 C196A loss in specific activity of 55% Drosophila melanogaster
1.2.1.24 C196S loss in specific activity of 74% Drosophila melanogaster
1.2.1.24 C243S loss in specific activity of 70% Drosophila melanogaster
1.2.1.24 C311A decreased SSADH activity to undetectable levels Drosophila melanogaster
1.2.1.24 C311S decreased SSADH activity to undetectable levels Drosophila melanogaster
1.2.1.24 C502S loss in specific activity of 93% Drosophila melanogaster
1.2.1.24 E277D leads to a product with similar specific activity Drosophila melanogaster
1.2.1.24 E277K charge inversion, which results in recombinant protein totally devoid of detectable SSADH activity Drosophila melanogaster
1.2.1.24 E277Q concomitant loss of the negative charge, which results in recombinant protein totally devoid of detectable SSADH activity Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.24 0.0047
-
succinate semialdehyde
-
Drosophila melanogaster
1.2.1.24 0.051
-
n-hexanal
-
Drosophila melanogaster
1.2.1.24 0.057
-
n-Pentanal
-
Drosophila melanogaster
1.2.1.24 0.0909
-
NAD+
-
Drosophila melanogaster
1.2.1.24 0.106
-
n-Butanal
-
Drosophila melanogaster
1.2.1.24 1.2
-
NADP+
-
Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.24 98200
-
x * 98200, SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.24 Drosophila melanogaster Q9VBP6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.24 by Ni2+-NTA agarose chromatography Drosophila melanogaster

Storage Stability

EC Number Storage Stability Organism
1.2.1.24 0°C, 100 mM sodium phosphate, pH 8.5, 1mM DTT, 43% glycerol, 3 years Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.24 m-carboxybenzaldehyde + NAD+ + H2O
-
Drosophila melanogaster m-carboxybenzoate + NADH + H+
-
?
1.2.1.24 m-methylcarboxybenzaldehyde + NAD+ + H2O
-
Drosophila melanogaster m-methylcarboxybenzoate + NADH + H+
-
?
1.2.1.24 additional information no activity with formaldehyde, o-nitrobenzaldehyde, o-carboxybenzaldehyde, o-tolualdehyde, p-tolualdehyde, o-methoxybenzaldehyde, p-methoxybenzaldehyde o-hydroxybenzaldehyde, m-hydroxybenzaldehyde, phenylacetaldehyde and glyoxylic acid Drosophila melanogaster ?
-
?
1.2.1.24 n-butanal + NAD+ + H2O
-
Drosophila melanogaster butanoate + NADH + H+
-
?
1.2.1.24 n-hexanal + NAD+ + H2O
-
Drosophila melanogaster hexanoate + NADH + H+
-
?
1.2.1.24 n-octanal + NAD+ + H2O
-
Drosophila melanogaster n-octanoate + NADH + H+
-
?
1.2.1.24 n-pentanal + NAD+ + H2O
-
Drosophila melanogaster n-pentanoate + NADH + H+
-
?
1.2.1.24 succinate semialdehyde + NAD(P)+ + H2O cysteine 311 and glutamic acid 277 are likely candidates for the active site residues directly involved in catalysis Drosophila melanogaster succinate + NAD(P)H + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.24 ? x * 98200, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
1.2.1.24 SSADH
-
Drosophila melanogaster
1.2.1.24 succinic semialdehyde dehydrogenase
-
Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.24 22
-
optimal expression of pMalc2x-DmSSADH in Escherichia coli TB1 Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.24 6 8.5 at pH 8.5 MalE-DmSSADH is 10fold more active than at pH 6.0 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.24 NAD+
-
Drosophila melanogaster
1.2.1.24 NADP+ is also accepted as coenzyme substrate but leads to 7fold lower reaction rates compared to NAD+ at saturating succinate semialdehyde concentrations Drosophila melanogaster