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Literature summary extracted from

  • Zhang, D.; Lax, A.R.; Raina, A.K.; Bland, J.M.
    Differential cellulolytic activity of native-form and C-terminal tagged-form cellulase derived from coptotermes formosanus and expressed in E. coli (2009), Insect Biochem. Mol. Biol., 39, 516-522.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 cloned and overexpressed in both native form (nCfEG) and C-terminal His-tagged form (tCfEG) in Escherichia coli Coptotermes formosanus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 additional information C-terminal His-tagged form (tCfEG) shows hydrolytic activity on cellulosic substrates Coptotermes formosanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 47090
-
calculated molecular weight, C-terminal His-tagged form Coptotermes formosanus
3.2.1.4 48160
-
calculated molecular weight, wild-type Coptotermes formosanus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Coptotermes formosanus Q9BLD2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 purified to near homogeneity, gel filtration Coptotermes formosanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
Coptotermes formosanus ?
-
?
3.2.1.4 cellobiose + H2O
-
Coptotermes formosanus ?
-
?
3.2.1.4 cellotetraose + H2O
-
Coptotermes formosanus ?
-
?
3.2.1.4 cellotriose + H2O
-
Coptotermes formosanus ?
-
?
3.2.1.4 additional information hydrolyzing internal beta-1,4-glycosidic bonds and resulting in a smear of polymers with different lengths. The hydrolytic products of tCfEG are one unit sugar less than those produced by nCfEG Coptotermes formosanus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cellulase
-
Coptotermes formosanus
3.2.1.4 CfEG3a
-
Coptotermes formosanus
3.2.1.4 endoglucanase
-
Coptotermes formosanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 37
-
assay at Coptotermes formosanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 37 52
-
Coptotermes formosanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
assay at Coptotermes formosanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 9
-
Coptotermes formosanus