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Literature summary extracted from

  • Lloyd, M.D.; Boardman, K.D.; Smith, A.; van den Brink, D.M.; Wanders, R.J.; Threadgill, M.D.
    Characterisation of recombinant human fatty aldehyde dehydrogenase: implications for Sjoegren-Larsson syndrome (2007), J. Enzyme Inhib. Med. Chem., 22, 584-590.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.48 medicine direct link between FALDH inactivation and disease pathology of Sjögren-Larsson syndrome caused by accumulation of alcohols Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.48 recombinant His6-FADH expressed in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.48 D245N NAD+ cosubstrate binding is occuring but catalytic reduction is diminished Homo sapiens
1.2.1.48 G185A disruption of NAD+ binding/utilisation Homo sapiens
1.2.1.48 G412R disruption of interaction of side-chains with substrate Homo sapiens
1.2.1.48 H411Y disruption of the key abstraction of the hydroxyl hydrogen Homo sapiens
1.2.1.48 Q445R substitution in the recombinant protein Homo sapiens
1.2.1.48 T184M disruption of NAD+ binding/utilisation Homo sapiens
1.2.1.48 T184R disruption of NAD+ binding/utilisation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.48 0.0038
-
decanal
-
Homo sapiens
1.2.1.48 0.0083
-
hexadecanal
-
Homo sapiens
1.2.1.48 0.0103
-
Tetradecanal
-
Homo sapiens
1.2.1.48 0.0136
-
dodecanal
-
Homo sapiens
1.2.1.48 0.02
-
octadecanal
-
Homo sapiens
1.2.1.48 0.023
-
farnesal
-
Homo sapiens
1.2.1.48 0.0343
-
(11Z)-hexadec-11-enal
-
Homo sapiens
1.2.1.48 0.18
-
NAD+
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.48 microsome
-
Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.48 57000
-
x * 57000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.48 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.48 recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.48 cis-11-hexadecenal + NAD+ + H2O
-
Homo sapiens cis-11-hexadecenoic acid + NADH + H+
-
?
1.2.1.48 decanal + NAD+ + H2O
-
Homo sapiens decanoic acid + NADH + H+
-
?
1.2.1.48 dodecanal + NAD+ + H2O
-
Homo sapiens dodecanoic acid + NADH + H+
-
?
1.2.1.48 farnesal + NAD+ + H2O
-
Homo sapiens farnesoic acid + NADH + H+
-
?
1.2.1.48 heptanal + NAD+ + H2O
-
Homo sapiens heptanoic acid + NADH + H+
-
?
1.2.1.48 hexadecanal + NAD+ + H2O
-
Homo sapiens hexadecanoic acid + NADH + H+
-
?
1.2.1.48 additional information catalytically important residues to be involved in alcohol and aldehyde oxidation are Gln-120, Glu-207, Cys-241, Phe-333, Tyr-410 and His-411 Homo sapiens ?
-
?
1.2.1.48 octadecanal + NAD+ + H2O
-
Homo sapiens octadecanoic acid + NADH + H+
-
?
1.2.1.48 octanal + NAD+ + H2O
-
Homo sapiens octanoic acid + NADH + H+
-
?
1.2.1.48 tetradecanal + NAD+ + H2O
-
Homo sapiens tetradecanoic acid + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.48 ? x * 57000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.2.1.48 FALDH
-
Homo sapiens
1.2.1.48 fatty aldehyde dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.48 35
-
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.48 0.86
-
Tetradecanal
-
Homo sapiens
1.2.1.48 0.93
-
farnesal
-
Homo sapiens
1.2.1.48 0.95
-
hexadecanal
-
Homo sapiens
1.2.1.48 1.28
-
NAD+
-
Homo sapiens
1.2.1.48 1.4
-
(11Z)-hexadec-11-enal
-
Homo sapiens
1.2.1.48 1.52
-
octadecanal
-
Homo sapiens
1.2.1.48 2.18
-
decanal
-
Homo sapiens
1.2.1.48 2.23
-
dodecanal
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.48 9.5
-
-
Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.48 NAD+
-
Homo sapiens