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Literature summary extracted from
- Luminal starch substrate "brake" on maltase-glucoamylase activity is located within the glucoamylase subunit (2008), J. Nutr., 138, 685-692.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | acarbose | inhibitory effect is 2 orders of magnitude higher for ntMGAM than for native MGAM | Homo sapiens |  |
| 3.2.1.3 | maltose | - |
Homo sapiens | |
| 3.2.1.3 | maltotetraose | - |
Homo sapiens | |
| 3.2.1.3 | maltotriose | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | additional information | - |
additional information | Km-value: 4.34 g/l with corn maltodextrin as a substrate for the N-terminal subunit of MGAM, Vmax: 7.51 U/mg with corn maltodextrin as a substrate for the N-terminal subunit of MGAM. Km-value: 7.01 g/l with alpha-limit dextrin as a substrate for the N-terminal subunit of MGAM, Vmax: 11.0 U/mg with alpha-limit dextrin as a substrate for the N-terminal subunit of MGAM. Km-value: 0.06 g/l with corn maltodextrin as a substrate for the immunoprecipitated maltase-glucoamylase. Km-value: 0.49 g/l with alpha-limit dextrin as a substrate for immunoprecipitated maltase-glucoamylase, Vmax: 11.0 U/mg with alpha-limit dextrin as a substrate for the immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 0.05 | - |
maltotetraose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 0.05 | - |
maltopentaose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 0.22 | - |
maltotriose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 0.58 | - |
maltose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 3 | - |
maltotetraose | ntMGAM, N-terminal subunit of MGAM, Vmax: 7.65 U/mg | Homo sapiens | |
| 3.2.1.3 | 4.6 | - |
maltotriose | ntMGAM, N-terminal subunit of MGAM, Vmax: 9.58 U/mg | Homo sapiens | |
| 3.2.1.3 | 6.66 | - |
maltopentaose | ntMGAM, N-terminal subunit of MGAM, Vmax: 6.97 U/mg | Homo sapiens | |
| 3.2.1.3 | 7.71 | - |
maltose | ntMGAM, N-terminal subunit of MGAM, Vmax: 8.26 U/mg | Homo sapiens | |
| 3.2.1.10 | additional information | - |
additional information | Km-value: 9.89 g/l with corn maltodextrin as a substrate for the immunoprecipitated human sucrase-isomaltase complex. Km-value: 5.78 g/l with alpha-limit dextrin as a substrate for the immunoprecipitated human sucrase-isomaltase complex. Km-value: 0.02 g/l with corn maltodextrin as a substrate for the supernatant after immunoprecipitation of sucrase-isomaltase complex (Sup-SI). Km-value: 0.29 g/l with alpha-limit dextrin as a substrate for the supernatant after immunoprecipitation of sucrase-isomaltase complex (Sup-SI) | Homo sapiens | |
| 3.2.1.10 | 3.6 | - |
maltopentose | IP-SI, immunoprecipitated human sucrase-isomaltase complex | Homo sapiens | |
| 3.2.1.10 | 3.79 | - |
maltotetrose | IP-SI, immunoprecipitated human sucrase-isomaltase complex | Homo sapiens | |
| 3.2.1.10 | 5.55 | - |
maltotriose | IP-SI, immunoprecipitated human sucrase-isomaltase complex | Homo sapiens | |
| 3.2.1.10 | 6.83 | - |
maltose | IP-SI, immunoprecipitated human sucrase-isomaltase complex | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | additional information | Homo sapiens | analysis of the basic catalytic properties of the N-terminal subunit of MGAM (ntMGAM) on the hydrolysis of glucan substrates and compared it with those of human native MGAM | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Homo sapiens | - |
- |
- |
| 3.2.1.10 | Homo sapiens | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | alpha-limit dextrin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.3 | maltodextrin + H2O | corn maltodextrin | Homo sapiens | ? | - |
? | |
| 3.2.1.3 | maltopentaose + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.3 | maltose + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.3 | maltotetraose + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.3 | maltotriose + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.3 | additional information | analysis of the basic catalytic properties of the N-terminal subunit of MGAM (ntMGAM) on the hydrolysis of glucan substrates and compared it with those of human native MGAM | Homo sapiens | ? | - |
? | |
| 3.2.1.3 | starch + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.10 | alpha-limit dextrin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.10 | maltodextrin + H2O | corn maltodextrin | Homo sapiens | ? | - |
? | |
| 3.2.1.10 | maltopentose + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.10 | maltose + H2O | - |
Homo sapiens | D-glucose + D-glucose | - |
? | |
| 3.2.1.10 | maltotetraose + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.2.1.10 | maltotriose + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.3 | glucoamylase | - |
Homo sapiens |
| 3.2.1.3 | maltase-glucoamylase | - |
Homo sapiens |
| 3.2.1.3 | MGAM | - |
Homo sapiens |
| 3.2.1.10 | sucrase-isomaltase complex | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 7 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 4 | 7 | - |
Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.3 | additional information | - |
additional information | Ki-value: 12.7 g/l with corn maltodextrin as a substrate for the immunoprecipitated maltase-glucoamylase. Ki-value: 37.6 g/l with alpha-limit dextrin as a substrate for immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 0.000074 | - |
acarbose | IP-MGAM is highly sensitive to acarbose with a calculated Ki of 74 nmol/l | Homo sapiens | |
| 3.2.1.3 | 0.24 | - |
maltotriose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 0.58 | - |
maltotetraose | IP-MGAM, immunoprecipitated maltase-glucoamylase | Homo sapiens | |
| 3.2.1.3 | 16 | - |
acarbose | ntMGAM had a relatively low sensitivity to acarbose | Homo sapiens | |
| 3.2.1.3 | 35 | - |
maltotriose | ntMGAM, N-terminal subunit of MGAM | Homo sapiens | |
| 3.2.1.3 | 67.6 | - |
maltotetraose | ntMGAM, N-terminal subunit of MGAM | Homo sapiens | |
| 3.2.1.3 | 86.9 | - |
maltose | ntMGAM, N-terminal subunit of MGAM | Homo sapiens | |
| 3.2.1.10 | additional information | - |
additional information | Ki-value: 13.1 g/l with corn maltodextrin as a substrate for the supernatant after immunoprecipitation of sucrase-isomaltase complex (Sup-SI). Ki-value: 70.5 g/l with alpha-limit dextrin as a substrate for the supernatant after immunoprecipitation of sucrase-isomaltase complex (Sup-SI) | Homo sapiens |
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