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Literature summary extracted from

  • Joshi, S.; Singh, A.R.; Kumar, A.; Misra, P.C.; Siddiqi, M.I.; Saxena, J.K.
    Molecular cloning and characterization of Plasmodium falciparum transketolase (2008), Mol. Biochem. Parasitol., 160, 32-41.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.2.1.1 drug development Plasmodium falciparum transketolase shows low homology with the host enzyme, thus may serve as a novel and promising target for designing inhibitors with anti-malarial activity Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 overexpressed in Escherichia coli BL21(DE3)Rosetta cells Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 p-hydroxyphenylpyruvate potent inhibitor when hydroxypyruvate is used as a substrate, whereas noncompetitive inhibition with fructose-6-phosphate Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 2.25
-
 fructose-6-phosphate
-
 Plasmodium falciparum
2.2.1.1 4.78
-
 Hydroxypyruvate
-
 Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.1 cytosol
-
 Plasmodium falciparum 5829
-
2.2.1.1 nucleus
-
 Plasmodium falciparum 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+
-
 Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.1 70000
-
 6 * 70000, SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Plasmodium falciparum
-
 clone NF54
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 on Ni2+-NTA resin and by ammonium sulfate precipitation Plasmodium falciparum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.2.1.1 additional information late trophozoite stage Plasmodium falciparum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 fructose-6-phosphate + hydroxypyruvate
-
 Plasmodium falciparum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.1 hexamer 6 * 70000, SDS-PAGE Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.1 0.305
-
 p-hydroxyphenylpyruvate with hydroxypyruvate as substrate Plasmodium falciparum