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Literature summary extracted from

  • Exposito, O.; Bonfill, M.; Onrubia, M.; Jane, A.; Moyano, E.; Cusido, R.M.; Palazon, J.; Pinol, M.T.
    Effect of taxol feeding on taxol and related taxane production in Taxus baccata suspension cultures (2008), N. Biotechnol., 25, 252-259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.17 taxol exogenous Taxol (paclitaxel) causes an considerable increase in taxadiene synthase activity, the expression level peaks at day 4 with a rapid decrease at day 8, reaching a second, lower, peak at day 12. By day 16, ts mRNA is only detectable in the untreated control at a very low level. The concentration of taxol that most effectively induces ts mRNA expression is 50 mg/l compared to the control, the transcript levels are 2.5-, 3.1- and 3.3fold higher at 4, 8 and 12 days of culture. Taxus baccata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.17 Mg2+ 1 mM is included in assay medium Taxus baccata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate Taxus baccata the first committed step toward taxanes and taxol biosynthesis involves taxadiene synthase which promotes the cyclization of geranylgeranyl diphosphate leading to the taxane skeleton taxa-4,11-diene + diphosphate the product of the biosynthetic step is not a limiting substrate in taxol formation ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.17 Taxus baccata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.17
-
 Taxus baccata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.17 cell culture
-
 Taxus baccata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.17 additional information
-
 a relatively high level of activity is observed at day 4, when it is 2.8-, 6.8-, 3.2- and 1.7fold higher, respectively, in the cultures treated with 25, 50, 100 and 200 mg/l taxol than in the untreated control (6.64 pkat/mg of protein). Enzyme activity then decreases markedly from days 4 to 12 and thereafter is measurable only at a very low level until day 20. Taxus baccata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate the first committed step toward taxanes and taxol biosynthesis involves taxadiene synthase which promotes the cyclization of geranylgeranyl diphosphate leading to the taxane skeleton Taxus baccata taxa-4,11-diene + diphosphate the product of the biosynthetic step is not a limiting substrate in taxol formation ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.17 taxadiene synthase
-
 Taxus baccata
4.2.3.17 TXS
-
 Taxus baccata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.17 32
-
 assay at Taxus baccata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.17 8.5
-
 assay at Taxus baccata