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Literature summary extracted from

  • Nashilevitz, S.; Melamed-Bessudo, C.; Aharoni, A.; Kossmann, J.; Wolf, S.; Levy, A.A.
    The legwd mutant uncovers the role of starch phosphorylation in pollen development and germination in tomato (2009), Plant J., 57, 1-13.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.4 amplified from young leave cDNA for sequencing Solanum lycopersicum
2.7.9.4 from pBluescript-GWD via pAA100-35S and pBinPlus to construct pLAT52::StGWD for expression in Micro-Tom tomato plants under control of a ‘late’ pollen-specific promoter following Agrobacterium-mediated transformation Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.4 plastid
-
 Solanum lycopersicum 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.4 ATP + alpha-glucan + H2O Solanum lycopersicum activity required for pollen tube germination AMP + phospho-alpha-glucan + phosphate
-
 ?
2.7.9.4 ATP + alpha-glucan + H2O Solanum lycopersicum activity required for pollen tube germination in male tomato plants the impairment of which is linked to starch accumulation and reduction in level of soluble sugars AMP + phospho-alpha-glucan + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.4 Solanum lycopersicum
-
 wild-type or legwd::Ds mutant plants with different genetic backgrounds: cv. Micro-Tom, cv. Ailsa Craig, cv. M82, cv. VF36
-
2.7.9.4 Solanum lycopersicum B5B3R3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.4 leaf
-
 Solanum lycopersicum
-
2.7.9.4 pollen in vitro germination at 25°C for 8 h (to 50% impaired in absence of functional GWD, starch accumulation and reduction in level of soluble sugars) Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.4 ATP + alpha-glucan + H2O activity required for pollen tube germination Solanum lycopersicum AMP + phospho-alpha-glucan + phosphate
-
 ?
2.7.9.4 ATP + alpha-glucan + H2O activity required for pollen tube germination in male tomato plants the impairment of which is linked to starch accumulation and reduction in level of soluble sugars Solanum lycopersicum AMP + phospho-alpha-glucan + phosphate
-
 ?
2.7.9.4 ATP + alpha-glucan + H2O 4.5fold accumulation of starch and 1.8fold reduction in level of soluble sugars in absence of functional GWD Solanum lycopersicum AMP + phospho-alpha-glucan + phosphate
-
 ?
2.7.9.4 ATP + alpha-glucan + H2O rescue of pollen germination in GWD-deficient mutant tomato plant Solanum lycopersicum AMP + phospho-alpha-glucan + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.9.4 alpha-glucan, water dikinase
-
 Solanum lycopersicum
2.7.9.4 alpha-glucan, water dikinase formerly termed R1 Solanum lycopersicum
2.7.9.4 GWD encoded by StGWD gene, locus Y09533 Solanum lycopersicum
2.7.9.4 GWD encoded by tomato LeGWD gene Solanum lycopersicum
2.7.9.4 LeGWD
-
 Solanum lycopersicum