EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.9 | APR, DNA and amino acid sequence determination and analysis, semiquantitative expression analysis, overexpression of His-tagged APR isozyme in Escherichia coli | Physcomitrium patens |
1.8.4.10 | APR-B, DNA and amino acid sequence determination and analysis, semiquantitative expression analysis, overexpression of His-tagged APR-B isozyme in Escherichia coli | Physcomitrium patens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.9 | additional information | construction of APR-B knockout plants, the mutant plants are able to grow on sulfate as a sole sulfur source, and the content of low molecular weight thiols is not different from wild-type plants. However, when treated with low concentrations of cadmium, the APR-B knockout plants are more sensitive than wild-type plants, phenotype, overview | Physcomitrium patens |
1.8.4.10 | additional information | construction of APR-B knockout plants, the mutant plants are able to grow on sulfate as a sole sulfur source, and the content of low molecular weight thiols is not different from wild-type plants. However, when treated with low concentrations of cadmium, the APR-B knockout plants are more sensitive than wild-type plants, phenotype, overview | Physcomitrium patens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.9 | Fe2+ | APR contains a FeS cluster | Physcomitrium patens | |
1.8.4.9 | Mg2+ | - |
Physcomitrium patens | |
1.8.4.10 | Mg2+ | - |
Physcomitrium patens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.9 | adenylyl sulfate + glutathione | Physcomitrium patens | - |
AMP + sulfite + glutathione disulfide | - |
? | |
1.8.4.10 | adenylyl sulfate + thioredoxin | Physcomitrium patens | in Physcomitrella patens, APS reduction is not the major control step of sulfate assimilation | AMP + sulfite + thioredoxin disulfide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.9 | Physcomitrium patens | - |
- |
- |
1.8.4.10 | Physcomitrium patens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.9 | adenylyl sulfate + DTT | - |
Physcomitrium patens | ? | - |
? | |
1.8.4.9 | adenylyl sulfate + glutathione | - |
Physcomitrium patens | AMP + sulfite + glutathione disulfide | - |
? | |
1.8.4.10 | adenylyl sulfate + thioredoxin | - |
Physcomitrium patens | AMP + sulfite + thioredoxin disulfide | - |
? | |
1.8.4.10 | adenylyl sulfate + thioredoxin | in Physcomitrella patens, APS reduction is not the major control step of sulfate assimilation | Physcomitrium patens | AMP + sulfite + thioredoxin disulfide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.9 | APR | - |
Physcomitrium patens |
1.8.4.9 | APS reductase | - |
Physcomitrium patens |
1.8.4.10 | APR-B | - |
Physcomitrium patens |
1.8.4.10 | APS reductase | - |
Physcomitrium patens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.9 | 37 | - |
assay at | Physcomitrium patens |
1.8.4.10 | 37 | - |
assay at | Physcomitrium patens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.9 | 9 | - |
assay at | Physcomitrium patens |
1.8.4.10 | 9 | - |
assay at | Physcomitrium patens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.9 | glutathione | - |
Physcomitrium patens | |
1.8.4.9 | additional information | APR contains a FeS cluster | Physcomitrium patens | |
1.8.4.10 | additional information | PpAPR-B does not contain the FeS cluster, which is believed to determine the substrate specificity of other APR enzymes from seed plants | Physcomitrium patens | |
1.8.4.10 | thioredoxin | - |
Physcomitrium patens |