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Literature summary extracted from

  • Baldwin, J.C.; Karthikeyan, A.S.; Cao, A.; Raghothama, K.G.
    Biochemical and molecular analysis of LePS2;1: a phosphate starvation induced protein phosphatase gene from tomato (2008), Planta, 228, 273-280.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expressed in Escherichia coli BL21 P-lysis S cells Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 additional information addition of EGTA and vanadate to the assay does not affect the enzyme activity Solanum lycopersicum
3.1.3.16 okadaic acid complete inhibition at 50 nM Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 additional information addition of Mg2+ and Ca2+ does not affect the enzyme activity Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Solanum lycopersicum
-
 cultivar VFNT Cherry
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 Ni-NTA column chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.16 leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 RRApTVA + H2O a protein concentration at 15 ng per reaction was found to be optimal for dephosphorylation of the synthetic peptide RRApTVA, maximum enzyme activity can be achieved in the presence of 100 ng of substrate Solanum lycopersicum RRATVA + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 PS2;1 protein plays an important role in phosphate starvation-induced processes in tomato Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 6
-
-
 Solanum lycopersicum