EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.99.2 | hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 0.1 M NaCl, 2.0 M ammonium sulfate and 15% (v/v) glycerol, at 4°C | Desulfovibrio vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.99.2 | 75000 | - |
1 * 75000 + 1 * ?, X-ray crystallography | Desulfovibrio vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.99.2 | Desulfovibrio vulgaris | B8PS61 and B8DS74 | alpha subunit and beta subunit | - |
1.8.99.2 | Desulfovibrio vulgaris Miyazaki F | B8PS61 and B8DS74 | alpha subunit and beta subunit | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.99.2 | DEAE-Toyopearl 650S column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration | Desulfovibrio vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.99.2 | adenylyl sulfate + reduced acceptor | - |
Desulfovibrio vulgaris | AMP + sulfite + acceptor | - |
? | |
1.8.99.2 | adenylyl sulfate + reduced acceptor | - |
Desulfovibrio vulgaris Miyazaki F | AMP + sulfite + acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.99.2 | heterodimer | 1 * 75000 + 1 * ?, X-ray crystallography | Desulfovibrio vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.99.2 | adenylylsulfate reductase | - |
Desulfovibrio vulgaris |
1.8.99.2 | APS reductase | - |
Desulfovibrio vulgaris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.99.2 | 4Fe-4S-center | contains two 4Fe-4S clusters in the beta-subunit | Desulfovibrio vulgaris | |
1.8.99.2 | FAD | contains a noncovalently bound FAD | Desulfovibrio vulgaris |