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Literature summary extracted from

  • Van Hellemond, E.; Vermote, L.; Koolen, W.; Sonke, T.; Zandvoort, E.; Heuts, D.; Janssen, D.; Fraaije, M.
    Exploring the biocatalytic scope of alditol oxidase from Streptomyces coelicolor (2009), Adv. Synth. Catal., 351, 1523-1530.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.3.4 synthesis AldO is an enantioselective biocatalyst for the kinetic resolution of racemic 1,2-diols Streptomyces coelicolor
1.1.3.41 synthesis AldO is an enantioselective biocatalyst for the kinetic resolution of racemic 1,2-diols Streptomyces coelicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.41 expressed in Escherichia coli Top10 cells Streptomyces coelicolor

General Stability

EC Number General Stability Organism
1.1.3.41 under conditions which are applied for the oxidation experiments (10 mM of aldoitol oxidase in 50 mM sodium phosphate buffer, pH 7.5, at 30°C and rotary shaking (100 rpm)) the enzyme has a half-life of 30 h Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 additional information
-
 additional information substrate specificity and steady state kinetics, overview Streptomyces coelicolor
1.1.3.4 0.32
-
 xylitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 1.4
-
 D-sorbitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 10
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 25
-
 L-Threitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 35
-
 2-amino-1-pentanol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 36
-
 D-mannitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 42
-
 4-pentene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 52
-
 1,2-pentanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 83
-
 1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 86
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 97
-
 1,2-hexanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 150
-
 1,2-Butanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 170
-
 1,2,4-butanetriol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 250
-
 3-butene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 350
-
 glycerol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 430
-
 L-arabinose pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 480
-
 3-butenol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 additional information
-
 additional information substrate specificity and steady state kinetics, overview Streptomyces coelicolor
1.1.3.41 0.32
-
 xylitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.32
-
 xylitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 1.4
-
 D-sorbitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 1.4
-
 D-sorbitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 10
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 25
-
 L-Threitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 25
-
 L-Threitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 35
-
 2-amino-1-pentanol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 35
-
 2-amino-1-pentanol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 36
-
 D-mannitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 36
-
 D-mannitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 42
-
 4-pentene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 42
-
 4-pentene-1,2-diol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 52
-
 1,2-pentanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 52
-
 1,2-pentanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 83
-
 1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 83
-
 1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 86
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 86
-
 (S)-1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 97
-
 1,2-hexanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 97
-
 1,2-hexanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 101
-
 (R)-1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 150
-
 1,2-Butanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 150
-
 1,2-Butanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 170
-
 1,2,4-butanetriol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 170
-
 1,2,4-butanetriol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 250
-
 3-butene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 250
-
 3-butene-1,2-diol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 350
-
 glycerol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 350
-
 glycerol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 430
-
 L-arabinose pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 430
-
 L-arabinose in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 480
-
 3-butenol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 480
-
 3-butenol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Streptomyces coelicolor
-
-
-
1.1.3.41 Streptomyces coelicolor
-
-
-
1.1.3.41 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.41
-
 Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.4 beta-D-glucose + O2 = D-glucono-1,5-lactone + H2O2 proposed mechanism for the oxidation of 1,2-diols to the alpha-hydroxy acids, overview Streptomyces coelicolor
a
1.1.3.41 an alditol + O2 = an aldose + H2O2 proposed mechanism for the oxidation of 1,2-diols to the alpha-hydroxy acids, overview Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 17.3
-
 substrate xylitol Streptomyces coelicolor
1.1.3.41 17.3
-
 substrate xylitol Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 (R)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 (R)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor (2R)-hydroxy(phenyl)ethanoic acid + H2O2 product identification by NMR ?
1.1.3.4 (S)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 1,2,4-butanetriol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 1,2-butanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 1,2-hexanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 1,2-pentanediol + O2
-
 Streptomyces coelicolor 2-hydroxypentanoate + H2O2 product identification by NMR ?
1.1.3.4 1,3-butanediol + O2
-
 Streptomyces coelicolor 2-hydroxypropanal + H2O2 product identification by GC-MS ?
1.1.3.4 1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor hydroxy(phenyl)ethanoic acid + H2O2
-
 r
1.1.3.4 2-amino-1-pentanol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 3-butene-1,2-diol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 3-butenol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 4-pentene-1,2-diol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 D-mannitol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 D-mannitol + O2
-
 Streptomyces coelicolor A3(2) ?
-
 ?
1.1.3.4 D-sorbitol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 D-sorbitol + O2
-
 Streptomyces coelicolor A3(2) ?
-
 ?
1.1.3.4 glycerol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 L-arabinose + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 L-arabinose + O2
-
 Streptomyces coelicolor A3(2) ?
-
 ?
1.1.3.4 L-threitol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.4 additional information substrate specificity,besides alditols, 1,2-diols are reasonable substrates indicating that two adjacent hydroxy groups at C-1 and C-2 seem to be a minimal requirement for a compound in order to be effectively oxidized by AldO, overview Streptomyces coelicolor ?
-
 ?
1.1.3.4 additional information substrate specificity,besides alditols, 1,2-diols are reasonable substrates indicating that two adjacent hydroxy groups at C-1 and C-2 seem to be a minimal requirement for a compound in order to be effectively oxidized by AldO, overview Streptomyces coelicolor A3(2) ?
-
 ?
1.1.3.4 xylitol + O2 best substrate Streptomyces coelicolor ?
-
 ?
1.1.3.4 xylitol + O2 best substrate Streptomyces coelicolor A3(2) ?
-
 ?
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor hydroxy(phenyl)acetic acid + H2O2 product identification by NMR ?
1.1.3.41 (R)-1-phenyl-1,2-ethanediol + O2 the enzyme is highly enantioselective for the oxidation of (R)-1-phenyl-1,2-ethanediol Streptomyces coelicolor mandelic acid + H2O2
-
 ?
1.1.3.41 (S)-1-phenyl-1,2-ethanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 1,2,4-butanetriol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 1,2-butanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 1,2-hexanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 1,2-pentanediol + O2
-
 Streptomyces coelicolor 2-hydroxypentanoic acid + H2O2 product identification by NMR ?
1.1.3.41 1,2-pentanediol + O2 50% conversion after 22 h Streptomyces coelicolor 2-hydroxypentanoic acid + H2O2
-
 ?
1.1.3.41 1,2-propanediol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 1,3,5-pentanetriol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 1,3-butanediol + O2
-
 Streptomyces coelicolor 3-hydroxybutanal + H2O2
-
 ?
1.1.3.41 1,3-butanediol + O2
-
 Streptomyces coelicolor 3-hydroxybutanal + H2O2 product identification by GC-MS ?
1.1.3.41 1,4-butanediol + O2 very poor substrate Streptomyces coelicolor ?
-
 ?
1.1.3.41 1-phenyl-1,2-ethanediol + O2 the enzyme is highly enantioselective for the oxidation of 1-phenyl-1,2-ethanediol, 35% conversion to mandelic acid and two minor by-products (less than 5%) is observed after 65 h Streptomyces coelicolor ?
-
 ?
1.1.3.41 2-amino-1-pentanol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 3-butene-1,2-diol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 3-butenol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 4-pentene-1,2-diol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 cis-2-butene-1,4-diol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 D-galactose + O2 very poor substrate Streptomyces coelicolor ?
-
 ?
1.1.3.41 D-mannitol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 D-sorbitol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 glycerol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 L-arabinose + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 L-arabinose + O2 very poor substrate Streptomyces coelicolor ?
-
 ?
1.1.3.41 L-threitol + O2
-
 Streptomyces coelicolor ?
-
 ?
1.1.3.41 additional information substrate specificity, besides alditols, 1,2-diols are reasonable substrates indicating that two adjacent hydroxy groups at C-1 and C-2 seem to be a minimal requirement for a compound in order to be effectively oxidized by AldO, overview Streptomyces coelicolor ?
-
 ?
1.1.3.41 additional information the enzyme is able to perform oxidations of alcohols into aldehydes/ketones (single oxidation) and oxidations of alcohols into acids (double oxidation) Streptomyces coelicolor ?
-
 ?
1.1.3.41 xylitol + O2 best substrate Streptomyces coelicolor ?
-
 ?
1.1.3.41 xylitol + O2 best substrate Streptomyces coelicolor D-xylose + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.41 AldO
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 55
-
-
 Streptomyces coelicolor
1.1.3.41 55
-
-
 Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 37
-
 half-life of 40 h Streptomyces coelicolor
1.1.3.4 50
-
 half-life of 4 h Streptomyces coelicolor
1.1.3.41 37
-
 half-life of 40 h Streptomyces coelicolor
1.1.3.41 37 50 the enzyme has a half-life of 40 h at 37°C and 4 h at 50°C Streptomyces coelicolor
1.1.3.41 50
-
 half-life of 4 h Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.4 0.008
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.017
-
 2-amino-1-pentanol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.1
-
 3-butenol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.29
-
 1,2-Butanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.34
-
 3-butene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.35
-
 4-pentene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.36
-
 1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.74
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 0.85
-
 1,2-pentanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 1.6
-
 glycerol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 1.7
-
 L-arabinose pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 2
-
 1,2-hexanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 4.4
-
 1,2,4-butanetriol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 6.3
-
 L-Threitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 9.2
-
 D-mannitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 13
-
 xylitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.4 17
-
 D-sorbitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.008
-
 (S)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.008
-
 (S)-1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.017
-
 2-amino-1-pentanol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.017
-
 2-amino-1-pentanol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.1
-
 3-butenol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.1
-
 3-butenol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.29
-
 1,2-Butanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.29
-
 1,2-Butanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.34
-
 3-butene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.34
-
 3-butene-1,2-diol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.35
-
 4-pentene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.35
-
 4-pentene-1,2-diol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.36
-
 1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.36
-
 1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.74
-
 (R)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.74
-
 (R)-1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.85
-
 1,2-pentanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 0.85
-
 1,2-pentanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 1.6
-
 glycerol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 1.6
-
 glycerol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 1.7
-
 L-arabinose pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 1.7
-
 L-arabinose in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 2
-
 1,2-hexanediol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 2
-
 1,2-hexanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 4.4
-
 1,2,4-butanetriol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 4.4
-
 1,2,4-butanetriol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 6.3
-
 L-Threitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 6.3
-
 L-Threitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 9.2
-
 D-mannitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 9.2
-
 D-mannitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 13
-
 xylitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 13
-
 xylitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 17
-
 D-sorbitol pH 7.5, 30°C Streptomyces coelicolor
1.1.3.41 17
-
 D-sorbitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 7.5
-
 assay at Streptomyces coelicolor
1.1.3.41 7.5
-
 assay at Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.4 6 9 over 80% of maximal activity within this range Streptomyces coelicolor
1.1.3.41 6 9
-
 Streptomyces coelicolor
1.1.3.41 6 9 over 80% of maximal activity within this range Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.41 0.0001
-
 (S)-1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0002
-
 3-butenol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0003
-
 D-galactose in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0003
-
 1,2-propanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0006
-
 2-amino-1-pentanol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0014
-
 3-butene-1,2-diol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0019
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 1,2-Butanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.004
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 L-arabinose in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0043
-
 1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0046
-
 glycerol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0073
-
 (R)-1-phenyl-1,2-ethanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0078
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 1,3,5-pentanetriol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.0083
-
 4-pentene-1,2-diol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.016
-
 1,2-pentanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.021
-
 1,2-hexanediol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.026
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 1,2,4-butanetriol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.25
-
 L-Threitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 0.26
-
 D-mannitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 12
-
 D-sorbitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor
1.1.3.41 41
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 xylitol in 50 mM sodium phosphate buffer, at pH 7.5 and 30°C Streptomyces coelicolor