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Literature summary extracted from

  • Takanashi, M.; Shiraki, M.; Saito, T.
    Characterization of a novel 3-hydroxybutyrate dehydrogenase from Ralstonia pickettii T1 (2009), Antonie van Leeuwenhoek, 95, 249-262.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 additional information BDH1 is abundant in cells grown on D-fructose, D-glucose, maltose, oxaloacetate, 2-oxoglutarate, L-alanine, L-arginine, L-methionine, poly-3-hydroxybutyrate, and nutrient broth, and poor in cells grown on pyruvate, gluconate, fumarate, L-malate, propionate, and n-butyrate Ralstonia pickettii
1.1.1.30 additional information BDH2 is abundant in cells grown on acetate, ethanol, D-arabinose, D-ribose, oxaloacetate, 2-oxoglutarate, malaete, L-histidine, L-alanine, L-arginine, L-methionine, and poly-3-hydroxybutyrate, and scant in cells grown on pyruvate, n-butyrate, and oleate Ralstonia pickettii
1.1.1.30 additional information BDH3 is produced only in cells grown on 3-hydroxybutyrate or poly-3-hydroxybutyrate as a carbon source. Expression of bdh3 may be coordinately regulated with a gene encoding putative 3-hydroxybutyrate permease Ralstonia pickettii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.30 BDH3 expressed in Escherichia coli BLR (DE3)/pLysS harboring pETT13 Ralstonia pickettii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.30 additional information the bdh1 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH1 causes a decline in the capacity to neutralize the stress Ralstonia pickettii
1.1.1.30 additional information the bdh2 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH2 causes a decline in the capacity to neutralize the stress Ralstonia pickettii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.30 Diamide
-
Ralstonia pickettii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.064
-
NAD+
-
Ralstonia pickettii
1.1.1.30 0.1
-
NAD+
-
Ralstonia pickettii
1.1.1.30 0.24
-
NADH
-
Ralstonia pickettii
1.1.1.30 0.25
-
acetoacetate
-
Ralstonia pickettii
1.1.1.30 0.31
-
NAD+
-
Ralstonia pickettii
1.1.1.30 0.32
-
NADH
-
Ralstonia pickettii
1.1.1.30 0.34
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1.1.30 0.49
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1.1.30 0.5
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1.1.30 1.1
-
NADH
-
Ralstonia pickettii
1.1.1.30 1.1
-
acetoacetate
-
Ralstonia pickettii
1.1.1.30 1.3
-
acetoacetate
-
Ralstonia pickettii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 28000
-
x * 28000, SDS-PAGE, BDH3 Ralstonia pickettii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Ralstonia pickettii A8R3J3 T1
-
1.1.1.30 Ralstonia pickettii Q2PEN2 T1
-
1.1.1.30 Ralstonia pickettii Q2PEN3 T1
-
1.1.1.30 Ralstonia pickettii T1 A8R3J3 T1
-
1.1.1.30 Ralstonia pickettii T1 Q2PEN2 T1
-
1.1.1.30 Ralstonia pickettii T1 Q2PEN3 T1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography Ralstonia pickettii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 1200
-
purified BDH3 Ralstonia pickettii

Storage Stability

EC Number Storage Stability Organism
1.1.1.30 -20°C, 20 mM phosphate buffer, pH 7.0, 20% glycerol Ralstonia pickettii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 D-3-hydroxybutyrate + NAD+ BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii acetoacetate + NADH + H+
-
r
1.1.1.30 D-3-hydroxybutyrate + NAD+ BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii acetoacetate + NADH + H+
-
r
1.1.1.30 D-3-hydroxybutyrate + NAD+ BDH3 is specialized to utilize 3-hydroxybutyrate Ralstonia pickettii acetoacetate + NADH + H+
-
r
1.1.1.30 D-3-hydroxybutyrate + NAD+ BDH3 is specialized to utilize 3-hydroxybutyrate Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r
1.1.1.30 D-3-hydroxybutyrate + NAD+ BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r
1.1.1.30 D-3-hydroxybutyrate + NAD+ BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r
1.1.1.30 poly-3-hydroxybutyrate + NAD+
-
Ralstonia pickettii acetoacetate + NADH + H+
-
r
1.1.1.30 poly-3-hydroxybutyrate + NAD+
-
Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 ? x * 28000, SDS-PAGE, BDH3 Ralstonia pickettii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.30 BDH1
-
Ralstonia pickettii
1.1.1.30 BDH2
-
Ralstonia pickettii
1.1.1.30 BDH3
-
Ralstonia pickettii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.30 58
-
NADH
-
Ralstonia pickettii
1.1.1.30 87
-
NAD+
-
Ralstonia pickettii
1.1.1.30 100
-
acetoacetate
-
Ralstonia pickettii
1.1.1.30 110
-
acetoacetate
-
Ralstonia pickettii
1.1.1.30 150
-
acetoacetate
-
Ralstonia pickettii
1.1.1.30 180
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1.1.30 280
-
NADH
-
Ralstonia pickettii
1.1.1.30 310
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1.1.30 340
-
NAD+
-
Ralstonia pickettii
1.1.1.30 360
-
NAD+
-
Ralstonia pickettii
1.1.1.30 500
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1.1.30 740
-
NADH
-
Ralstonia pickettii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.30 6.5 8.5 optimal pH of BDH3 is 8.5 in the oxidation reaction and 6.5 in the reduction reaction Ralstonia pickettii