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Literature summary extracted from

  • Inouye, S.; Sahara, Y.
    Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps (2008), Biochem. Biophys. Res. Commun., 365, 96-101.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.5 CaCl2 0.1 mM Gaussia princeps

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 expression in Escherichia coli BL21 Gaussia princeps

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.5 pCold-hGL two repeat sequences, two catalytic domains Gaussia princeps
1.13.12.5 pCold-hGL-27/97 one repeat sequence, with only one catalytic domain Gaussia princeps
1.13.12.5 pCold-hGL-98/168 one repeat sequence, with only one catalytic domain Gaussia princeps
1.13.12.5 pCold-hSGL with a signal peptide sequence for secretion Gaussia princeps

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.5 2-mercaptoethanol 0.01% Gaussia princeps
1.13.12.5 CuCl2 1 microM, inhibition of 95% Gaussia princeps
1.13.12.5 dithiothreitol 1 mM Gaussia princeps

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.12.5 additional information insoluble fraction in Escherichia coli cells Gaussia princeps
-
-
1.13.12.5 soluble fraction in Escherichia coli cells Gaussia princeps
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.5 Br- stimulates luminescence activity Gaussia princeps
1.13.12.5 Cl- stimulates luminescence activity Gaussia princeps
1.13.12.5 Cu2+ strong inhibition of luminescence activity Gaussia princeps
1.13.12.5 I- stimulates luminescence activity Gaussia princeps

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.5 10350
-
MALDI-TOF-MS analyses, hGL-98/168 Gaussia princeps
1.13.12.5 10740
-
MALDI-TOF-MS analyses, hGL-27/97 Gaussia princeps
1.13.12.5 20020
-
MALDI-TOF-MS analyses, hGL Gaussia princeps
1.13.12.5 25000
-
SDS-PAGE, pCold-hGL Gaussia princeps

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Gaussia princeps
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5 souble and insoluble fraction by nickel-chelate column Gaussia princeps

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.5 additional information
-
1170000000 relative light units/mg, hGL-27/97, soluble fraction Gaussia princeps
1.13.12.5 additional information
-
14220000000 relative light units/mg, hGL, insoluble fraction Gaussia princeps
1.13.12.5 additional information
-
20000000 relative light units/mg, hGL-98/168, insoluble fraction Gaussia princeps
1.13.12.5 additional information
-
3120000000 relative light units/mg, hGL-27/97, insoluble fraction Gaussia princeps
1.13.12.5 additional information
-
51230000000 relative light units/mg, hGL, soluble fraction Gaussia princeps
1.13.12.5 additional information
-
590000000 relative light units/mg, hGL-98/168, soluble fraction Gaussia princeps

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 bis-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 coelenterazine + O2 0.5 microg, assay at pH 7.6, high substrate specificity for coelenterazine Gaussia princeps coelenteramide + CO2 + hnu
-
?
1.13.12.5 cp-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 e-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 f-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 fcp-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 h-coelenterazine + O2 assay at pH 7.6m, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 hcp-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 MeO-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?
1.13.12.5 n-coelenterazine + O2 assay at pH 7.6, about 100fold reduced relative luminescence Gaussia princeps hnu + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 Gaussia luciferase
-
Gaussia princeps

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.5 7.5
-
-
Gaussia princeps