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Literature summary extracted from

  • Johannes, J.; Unciuleac, M.C.; Friedrich, T.; Warkentin, E.; Ermler, U.; Boll, M.
    Inhibitors of the molybdenum cofactor containing 4-hydroxybenzoyl-CoA reductase (2008), Biochemistry, 47, 4964-4972.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.7.1 the structures of dithionite- and azide-bound 4-HBCR are solved at 2.1 and 2.2 A, respectively Thauera aromatica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.7.1 azide previously reported, not confirmed Thauera aromatica
1.1.7.1 cyanide cyanide inhibition occurs in both the oxidized and reduced state of 4-HBCR, in the reduced state 4-HBCR is reactivated by simple oxidation, in the oxidized state reactivation is only achieved in the presence of sulfide Thauera aromatica
1.1.7.1 Dithionite reactivation by oxidation Thauera aromatica
1.1.7.1 titanium(III)citrate irreversible inhibition Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.7.1 270000
-
-
 Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.7.1 benzoyl-CoA + oxidized ferredoxin + H2O Thauera aromatica
-
 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?
1.1.7.1 benzoyl-CoA + oxidized ferredoxin + H2O Thauera aromatica K172
-
 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.7.1 Thauera aromatica
-
-
-
1.1.7.1 Thauera aromatica K172
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.7.1 from extracts of Thauera aromatica, three chromatographic steps, using anion exchange chromatography on DEAE-Sepharose, Hi Load Q-Sepharose and chromatography on Cibacron Blue agarose Thauera aromatica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.7.1 4
-
 60 mg of enriched 4-HBCR is maximally obtained from 200 g of cells with specific activities of 3-5 micromol of 4-OH-BCoA reduced min-1 mg-1 Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.7.1 benzoyl-CoA + oxidized ferredoxin + H2O
-
 Thauera aromatica 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?
1.1.7.1 benzoyl-CoA + oxidized ferredoxin + H2O
-
 Thauera aromatica K172 4-hydroxybenzoyl-CoA + reduced ferredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.7.1 oligomer (alphabetagamma)2 subunit architecture Thauera aromatica

Synonyms

EC Number Synonyms Comment Organism
1.1.7.1 4-HBCR
-
 Thauera aromatica
1.1.7.1 4-hydroxybenzoyl-CoA reductase
-
 Thauera aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.7.1 7
-
 activity assay Thauera aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.7.1 FAD
-
 Thauera aromatica
1.1.7.1 Ferredoxin
-
 Thauera aromatica
1.1.7.1 iron-sulfur centre
-
 Thauera aromatica
1.1.7.1 molybdopterin cytosine dinucleotide
-
 Thauera aromatica