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Literature summary extracted from

  • Puri, N.; Gaur, M.; Sharma, M.; Shukla, S.; Ambudkar, S.V.; Prasad, R.
    The amino acid residues of transmembrane helix 5 of multidrug resistance protein CaCdr1p of Candida albicans are involved in substrate specificity and drug transport (2009), Biochim. Biophys. Acta, 1788, 1752-1761.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.2 overexpression of Cdr1p as a GFP-tagged protein in plasma membrane of Saccharomyces cerevisiae Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.2 A666G the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs, the mutant displays a hyperresistant phenotype towards fluconazole comparable to wild type Candida albicans
7.6.2.2 F673A the mutant shows a reduction in both Km and Vmax values of ATPase activity when compared to wild-type Cdr1p-GFP, and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 G672A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 I675A the mutant shows a reduction in both Km and Vmax values of ATPase activity when compared to wild-type Cdr1p-GFP, and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 L663A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 L664A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 M667A the mutant shows a reduction in both Km and Vmax values of ATPase activity when compared to wild-type Cdr1p-GFP, and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 additional information substrate specificities of transmembrane segment mutants, overview Candida albicans
7.6.2.2 P659A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 P676A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 P678A the mutant shows a reduction in both Km and Vmax values of ATPase activity when compared to wild-type Cdr1p-GFP, and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 T671A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 T677A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 V668A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans
7.6.2.2 V674A the mutant shows an uncoupling of ATPase and drug transport and displays a selective loss of resistance to one or more of the tested drugs Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.2 1.58
-
 ATP pH 7.5, 30°C, recombinant mutant I675A Candida albicans
7.6.2.2 2.68
-
 ATP pH 7.5, 30°C, recombinant mutant M667A Candida albicans
7.6.2.2 2.7
-
 ATP pH 7.5, 30°C, recombinant mutant P678A Candida albicans
7.6.2.2 3.62
-
 ATP pH 7.5, 30°C, recombinant mutant L663A Candida albicans
7.6.2.2 3.62
-
 ATP pH 7.5, 30°C, recombinant mutant Y670A Candida albicans
7.6.2.2 3.73
-
 ATP pH 7.5, 30°C, recombinant mutant L665A Candida albicans
7.6.2.2 3.92
-
 ATP pH 7.5, 30°C, recombinant mutant P673A Candida albicans
7.6.2.2 4.04
-
 ATP pH 7.5, 30°C, recombinant mutant I669A Candida albicans
7.6.2.2 4.06
-
 ATP pH 7.5, 30°C, recombinant mutant L664A Candida albicans
7.6.2.2 4.36
-
 ATP pH 7.5, 30°C, recombinant mutant T671A Candida albicans
7.6.2.2 4.75
-
 ATP pH 7.5, 30°C, recombinant mutant V674A Candida albicans
7.6.2.2 4.82
-
 ATP pH 7.5, 30°C, recombinant mutant P659A Candida albicans
7.6.2.2 4.83
-
 ATP pH 7.5, 30°C, recombinant mutant P676A Candida albicans
7.6.2.2 4.9
-
 ATP pH 7.5, 30°C, recombinant mutant T677A Candida albicans
7.6.2.2 5.21
-
 ATP pH 7.5, 30°C, recombinant mutant G672A Candida albicans
7.6.2.2 5.69
-
 ATP pH 7.5, 30°C, recombinant mutant V668A Candida albicans
7.6.2.2 5.71
-
 ATP pH 7.5, 30°C, recombinant mutant V662A Candida albicans
7.6.2.2 6.08
-
 ATP pH 7.5, 30°C, recombinant mutant A666G Candida albicans
7.6.2.2 6.39
-
 ATP pH 7.5, 30°C, recombinant wild-type enzyme Candida albicans
7.6.2.2 7.19
-
 ATP pH 7.5, 30°C, recombinant mutant A660G Candida albicans
7.6.2.2 7.55
-
 ATP pH 7.5, 30°C, recombinant mutant T658A Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.2 membrane transmembrane protein Candida albicans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.2 Mg2+
-
 Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.2 ATP + H2O + xenobiotic/in Candida albicans importance of Candida drug resistance protein Cdr1p of pathogenic Candida albicans in azole resistance ADP + phosphate + xenobiotic/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.2 Candida albicans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6.2.2 additional information
-
 substrate specificities of transmembrane segment mutants, overview Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.2 ATP + H2O + anisomycin/in
-
 Candida albicans ADP + phosphate + anisomycin/out
-
 ?
7.6.2.2 ATP + H2O + beta-estradiol/in
-
 Candida albicans ADP + phosphate + beta-estradiol/out
-
 ?
7.6.2.2 ATP + H2O + cycloheximide /in
-
 Candida albicans ADP + phosphate + cycloheximide /out
-
 ?
7.6.2.2 ATP + H2O + fluconazole/in
-
 Candida albicans ADP + phosphate + fluconazole/out
-
 ?
7.6.2.2 ATP + H2O + itraconazole/in
-
 Candida albicans ADP + phosphate + itraconazole/out
-
 ?
7.6.2.2 ATP + H2O + ketoconazole/in
-
 Candida albicans ADP + phosphate + ketoconazole/out
-
 ?
7.6.2.2 ATP + H2O + miconazole/in
-
 Candida albicans ADP + phosphate + miconazole/out
-
 ?
7.6.2.2 ATP + H2O + oligomycin/in
-
 Candida albicans ADP + phosphate + oligomycin/out
-
 ?
7.6.2.2 ATP + H2O + progesterone/in
-
 Candida albicans ADP + phosphate + progesterone/out
-
 ?
7.6.2.2 ATP + H2O + rhodamine 6G/in
-
 Candida albicans ADP + phosphate + rhodamine 6G/out
-
 ?
7.6.2.2 ATP + H2O + xenobiotic/in
-
 Candida albicans ADP + phosphate + xenobiotic/out
-
 ?
7.6.2.2 ATP + H2O + xenobiotic/in importance of Candida drug resistance protein Cdr1p of pathogenic Candida albicans in azole resistance Candida albicans ADP + phosphate + xenobiotic/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.2 Cdr1p
-
 Candida albicans
7.6.2.2 multidrug resistance protein
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.6.2.2 30
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6.2.2 7.5
-
 assay at Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
7.6.2.2 ATP
-
 Candida albicans