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Literature summary extracted from

  • Reca, I.B.; Brutus, A.; DAvino, R.; Villard, C.; Bellincampi, D.; Giardina, T.
    Molecular cloning, expression and characterization of a novel apoplastic invertase inhibitor from tomato (Solanum lycopersicum) and its use to purify a vacuolar invertase (2008), Biochimie, 90, 1611-1623.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 invertase inhibitor of Solanum lycopersicum 6.7 nM tomato invertase with 1-2.5 nM inhibitor retains more than 90% invertase activity, 3-4 nM less than 90% activity, 5 nM about 75%, about 6.7 nM about 60% activity, pH 4.9, 37 degrees Celsius, 100 mM sucrose as substrate, proteinaceous functional inhibitor is expressed in leaves, flowers, and green fruit, proteolytically cleaving the invertase Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 vacuole
-
 Solanum lycopersicum 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 60000
-
 native PAGE, SDS-PAGE produced only fragments of 46.3, 27.7, 18.6, and 16.8 kDa, in red fruits additionally a 44 kDa fragment, and a 35 kDa fragment shown to be produced by in planta proteolysis of invertase Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Solanum lycopersicum P29000
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein
-
 Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 green and red tomato fruits homogenized separately in 10 mM NaOAc cold buffer, pH 4.6 containing 1 M NaCl, centrifuged, supernatant precipitated, resuspended in buffer, dialyzed, loaded onto invertase inhibitor of Solanum lycopersicum affinity column, washing with buffer, eluted with 50 mM potassium phosphate buffer, pH 7.5, fractions with invertase activity pooled, loaded onto Hiload 16/60 Superdex 75 column, eluted with 20 mM Na acetate, pH 4.6, containing 150 mM NaCl Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 fruit
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranose + H2O sucrose, 0.2 M sodium acetate buffer, pH 4.9, 37°C Solanum lycopersicum D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 monomer glycosylated monomer composed of several splitting fragments that have to be tightly associated for enzyme activity to occur Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 TIV-1
-
 Solanum lycopersicum
3.2.1.26 Vacuolar invertase
-
 Solanum lycopersicum