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Literature summary extracted from

  • Chiku, K.; Uzawa, J.; Seki, H.; Amachi, S.; Fujii, T.; Shinoyama, H.
    Characterization of a novel polyphenol-specific oligoxyloside transfer reaction by a family 11 xylanase from Bacillus sp. KT12 (2008), Biosci. Biotechnol. Biochem., 72, 2285-2293.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 additional information the Bacillus sp. KT12 xylanolytic enzyme is a suitable enzyme for the synthesis of polyphenyl beta-oligoxylosides Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ba2+ inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 Ca2+ completely inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 Co2+ inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 Cu2+ inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 EDTA completely inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 Mg2+ inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 Mn2+ inhibits hydrolysis activity Bacillus subtilis
3.2.1.8 additional information hydrolytic activity almost entirely inhibits at acidic pH Bacillus subtilis
3.2.1.8 Zn2+ completely inhibits hydrolysis activity Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Fe2+ strongly activates transxylosylation activity Bacillus subtilis
3.2.1.8 additional information other metalions than Fe2+ have an insignificant effect on transxylosylation activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 20000
-
 1 * 20000, SDS-PAGE and HPLC Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus niger
-
-
-
3.2.1.8 Aspergillus niger IFO 31628
-
-
-
3.2.1.8 Bacillus subtilis Q45VU2
-
-
3.2.1.8 Bacillus subtilis KT12 Q45VU2
-
-
3.2.1.8 Penicillium expansum
-
-
-
3.2.1.8 Penicillium expansum IFO 8800
-
-
-
3.2.1.8 Trichoderma viride
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 by ammonium sulfate fractionation, centrifugation and gel filtration, 7.7fold Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-methoxyphenol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 ascorbic acid + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 beech wood xylan + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.8 beech wood xylan + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.8 beech wood xylan + H2O
-
 Penicillium expansum ?
-
 ?
3.2.1.8 beech wood xylan + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 beech wood xylan + H2O
-
 Penicillium expansum IFO 8800 ?
-
 ?
3.2.1.8 beech wood xylan + H2O
-
 Aspergillus niger IFO 31628 ?
-
 ?
3.2.1.8 caffeic acid + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 catechin + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 catechol + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 DOPA + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 dopamine + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 fructose + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 gallic acid + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 glucose + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 glucose + H2O weak transxylosylation activity Bacillus subtilis KT12 ?
-
 ?
3.2.1.8 hydroquinone + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 lactose + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 lactose + H2O strong transxylosylation activity Bacillus subtilis KT12 ?
-
 ?
3.2.1.8 maltose + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 maltose + H2O strong transxylosylation activity Bacillus subtilis KT12 ?
-
 ?
3.2.1.8 mannitol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 additional information displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol) Bacillus subtilis ?
-
 ?
3.2.1.8 additional information displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol) Bacillus subtilis KT12 ?
-
 ?
3.2.1.8 nutrient broth + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 phloroglucinol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 pyrogallol + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 resorcinol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 starch + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 sucrose + H2O strong transxylosylation activity Bacillus subtilis D-fructose + D-glucose
-
 ?
3.2.1.8 sucrose + H2O strong transxylosylation activity Bacillus subtilis KT12 D-fructose + D-glucose
-
 ?
3.2.1.8 xylooligosaccharide + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
3.2.1.8 xylose + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 20000, SDS-PAGE and HPLC Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Aspergillus niger
3.2.1.8 endoxylanase
-
 Trichoderma viride
3.2.1.8 endoxylanase
-
 Penicillium expansum
3.2.1.8 endoxylanase
-
 Bacillus subtilis
3.2.1.8 xylanase
-
 Aspergillus niger
3.2.1.8 xylanase
-
 Trichoderma viride
3.2.1.8 xylanase
-
 Penicillium expansum
3.2.1.8 xylanase
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 60 transxylosylation and hydrolysis activities are optimal at temperatures of 60°C and 40°C, respectively Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4 11 near constant transxylosylation activity Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Bacillus subtilis IEF-PAGE
-
 7.5