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Literature summary extracted from

  • Czerwiec, E.; Begley, G.S.; Bronstein, M.; Stenflo, J.; Taylor, K.; Furie, B.C.; Furie, B.
    Expression and characterization of recombinant vitamin K-dependent gamma-glutamyl carboxylase from an invertebrate, Conus textile (2002), Eur. J. Biochem., 269, 6162-6172.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.90 expression in COS cells or expressed in Sf21 insect cells Conus textile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.90 0.0017
-
28-residue peptides based on residues -18 to +10 of human proprothrombin pH 7.0 Conus textile
4.1.1.90 0.006
-
proFactor IX pH 7.0 Conus textile
4.1.1.90 0.052
-
reduced vitamin K1 pH 7.0 Conus textile
4.1.1.90 0.074
-
precursor analog containing 29 amino acids of the propeptide region pH 7.0 Conus textile
4.1.1.90 0.075
-
precursor analog containing 12 of the propeptide region pH 7.0 Conus textile
4.1.1.90 0.42
-
FLEEL pH 7.0 Conus textile
4.1.1.90 0.565
-
conotoxin epsilon-TxIX pH 7.0 Conus textile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.90 33000
-
cells transfected with the CAT-V5/His plasmid, Western Blot Conus textile
4.1.1.90 130000
-
SDS-PAGE, Sf21 cells transfected with the carboxylase cDNA-containing plasmid, Western Blot Conus textile

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Conus textile Q8IA33
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 28-residue peptides based on residues -18 to +10 of human proprothrombin + CO2 + O2 + reduced vitamin K1
-
Conus textile ?
-
?
4.1.1.90 conotoxin epsilon-TxIX + CO2 + O2 + vitamin KH2
-
Conus textile ?
-
?
4.1.1.90 e-TxIX12 + CO2 + O2 + vitamin KH2 residues 1-12 of e-TxIX Conus textile ?
-
?
4.1.1.90 FLEEL + CO2 + O2 + reduced vitamin K1
-
Conus textile ?
-
?
4.1.1.90 FLEEL + CO2 + O2 + vitamin KH2
-
Conus textile ? + vitamin K epoxide + H2O
-
?
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
Conus textile gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
?
4.1.1.90 additional information amino-acid sequences of the synthetic substrates and propeptides are shown Conus textile ?
-
?
4.1.1.90 precursor analog containing 12 of the propeptide region + CO2 + O2 + reduced vitamin K1
-
Conus textile ?
-
?
4.1.1.90 precursor analog containing 29 amino acids of the propeptide region + CO2 + O2 + reduced vitamin K1
-
Conus textile ?
-
?
4.1.1.90 pro-e-TxIX/12 + CO2 + O2 + vitamin KH2 residues -12 to -1 of e-TxIX precursor Conus textile ?
-
?
4.1.1.90 pro-e-TxIX/24 + CO2 + O2 + vitamin KH2 residues -12 to +12 of e-TxIX precursor Conus textile ?
-
?
4.1.1.90 pro-e-TxIX/41 + CO2 + O2 + vitamin KH2 residues -29 to +12 of e-TxIX precursor Conus textile ?
-
?
4.1.1.90 proFactor IX + CO2 + O2 + reduced vitamin K1
-
Conus textile ?
-
?
4.1.1.90 proFIX18 + CO2 + O2 + vitamin KH2 residues -18 to -1 of proFactor IX Conus textile ?
-
?
4.1.1.90 proFIX28 + CO2 + O2 + vitamin KH2 residues -18 to +10 of proFactor IX Conus textile ?
-
?
4.1.1.90 proPT18 + CO2 + O2 + vitamin KH2 residues -18 to -1 of proprothrombin. 28-residue peptides based on residues -18 to +10 of human proprothrombin and proFactor IX with Km values of 420 lM, 1.7 microM and 6 microM Conus textile ?
-
?
4.1.1.90 proPT28 + CO2 + O2 + vitamin KH2 residues -18 to +10 of proprothrombin Conus textile ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 gamma-glutamyl carboxylase
-
Conus textile
4.1.1.90 glutamate carboxylase
-
Conus textile

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K1
-
Conus textile