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Literature summary extracted from

  • Watanabe, S.; Piyanart, S.; Makino, K.
    Metabolic fate of L-lactaldehyde derived from an alternative L-rhamnose pathway (2008), FEBS J., 275, 5139-5149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.22 overexpression in Escherichia coli cells as a His6-tagged enzyme Azotobacter vinelandii
1.2.1.22 overexpression in Escherichia coli cells as a His6-tagged enzyme Scheffersomyces stipitis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.22 mitochondrion
-
 Scheffersomyces stipitis 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.22 K+
-
 Scheffersomyces stipitis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.22 55000
-
 SDS-PAGE Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD(P)+ + H2O Scheffersomyces stipitis
-
 (S)-lactate + NAD(P)H + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O Azotobacter vinelandii
-
 (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O Azotobacter vinelandii NBRC 102612
-
 (S)-lactate + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Azotobacter vinelandii
-
-
-
1.2.1.22 Azotobacter vinelandii NBRC 102612
-
-
-
1.2.1.22 Scheffersomyces stipitis
-
-
-
1.2.1.22 Scheffersomyces stipitis A3LNE3
-
-
1.2.1.22 Scheffersomyces stipitis A3M013
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.22 nickel-chelating affinity column Azotobacter vinelandii
1.2.1.22 nickel-chelating affinity column Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.22 0.355
-
 purified recombinant enzyme, S-lactaldehyde, NADP+ Scheffersomyces stipitis
1.2.1.22 1.65
-
 purified recombinant enzyme, (S)-lactaldehyde, NADP+ Scheffersomyces stipitis
1.2.1.22 2.1
-
 purified recombinant enzyme, R-lactaldehyde, NAD+ Scheffersomyces stipitis
1.2.1.22 2.28
-
 purified recombinant enzyme, (R)-D-lactaldehyde, NAD+ Azotobacter vinelandii
1.2.1.22 3.64
-
 purified recombinant enzyme, S-lactaldehyde, NAD+ Scheffersomyces stipitis
1.2.1.22 4.28
-
 purified recombinant enzyme, glycolaldehyde, NAD+ Scheffersomyces stipitis
1.2.1.22 4.43
-
 purified recombinant enzyme, (R)-lactaldehyde, NAD+ Scheffersomyces stipitis
1.2.1.22 6.95
-
 purified recombinant enzyme, (S)-lactaldehyde, NAD+ Scheffersomyces stipitis
1.2.1.22 8.94
-
 purified recombinant enzyme, glycolaldehyde, NAD+ Scheffersomyces stipitis
1.2.1.22 11.5
-
 purified recombinant enzyme, glycolaldehyde, NAD+ Azotobacter vinelandii
1.2.1.22 17.2
-
 purified recombinant enzyme, (S)-lactaldehyde, NAD+ Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 (R)-lactaldehyde + NAD+ + H2O 54% of the activity of L-lactaldehyde Scheffersomyces stipitis (R)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (R)-lactaldehyde + NAD+ + H2O 75% of the activity of (S)-lactaldehyde Scheffersomyces stipitis (R)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (R)-lactaldehyde + NAD+ + H2O 8.6% of the activity of (S)-lactaldehyde Azotobacter vinelandii (R)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (R)-lactaldehyde + NAD+ + H2O 8.6% of the activity of (S)-lactaldehyde Azotobacter vinelandii NBRC 102612 (R)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD(P)+ + H2O
-
 Scheffersomyces stipitis (S)-lactate + NAD(P)H + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O best substrate Azotobacter vinelandii (S)-lactate + NADH + H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O best substrate Scheffersomyces stipitis (S)-lactate + NADH + H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O best substrate Azotobacter vinelandii NBRC 102612 (S)-lactate + NADH + H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Azotobacter vinelandii (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Azotobacter vinelandii NBRC 102612 (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.22 glycolaldehyde + NAD+ + H2O
-
 Azotobacter vinelandii glycolate + NADH + 2 H+
-
 ?
1.2.1.22 glycolaldehyde + NAD+ + H2O
-
 Scheffersomyces stipitis glycolate + NADH + 2 H+
-
 ?
1.2.1.22 glycolaldehyde + NAD+ + H2O
-
 Azotobacter vinelandii NBRC 102612 glycolate + NADH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.22 monomer 1 * 55000, SDS-PAGE Scheffersomyces stipitis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.22 AvLADH
-
 Azotobacter vinelandii
1.2.1.22 L-lactaldehyde dehydrogenase
-
 Azotobacter vinelandii
1.2.1.22 L-lactaldehyde dehydrogenase
-
 Scheffersomyces stipitis
1.2.1.22 LADH
-
 Azotobacter vinelandii
1.2.1.22 LADH
-
 Scheffersomyces stipitis
1.2.1.22 PsLADH
-
 Scheffersomyces stipitis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 additional information no activity with NADP+ Azotobacter vinelandii
1.2.1.22 NAD+
-
 Azotobacter vinelandii
1.2.1.22 NAD+
-
 Scheffersomyces stipitis
1.2.1.22 NADP+
-
 Scheffersomyces stipitis