Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dopitova, R.; Mazura, P.; Janda, L.; Chaloupkova, R.; Jerabek, P.; Damborsky, J.; Filipi, T.; Kiran, N.S.; Brzobohaty, B.
    Functional analysis of the aglycone-binding site of the maize beta-glucosidase Zm-p60.1 (2008), FEBS J., 275, 6123-6135.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 F193A increase in affinity for a small polar aglycone, deep decrease in kcat value. 7.8% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 3% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays
3.2.1.21 F193A/F200K/W373K/F461L almost complete loss of catalytic activity, extensive alterations in strucutre Zea mays
3.2.1.21 F200K 1.1% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 0.3% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays
3.2.1.21 F461L 120% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 119% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays
3.2.1.21 W373K large decrease in kcat value. 0.5% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 0.04% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.045
-
4-nitrophenyl-beta-D-glucoside mutant F193A Zea mays
3.2.1.21 0.12
-
4-methylumbelliferyl-beta-D-glucoside mutant F193A Zea mays
3.2.1.21 0.148
-
4-methylumbelliferyl-beta-D-glucoside wild-type Zea mays
3.2.1.21 0.164
-
4-methylumbelliferyl-beta-D-glucoside mutant F461L Zea mays
3.2.1.21 0.65
-
4-nitrophenyl-beta-D-glucoside mutant F461L Zea mays
3.2.1.21 0.68
-
4-nitrophenyl-beta-D-glucoside wild-type Zea mays
3.2.1.21 1.51
-
4-methylumbelliferyl-beta-D-glucoside mutant F200K Zea mays
3.2.1.21 1.73
-
4-methylumbelliferyl-beta-D-glucoside mutant W373K Zea mays
3.2.1.21 2.1
-
4-nitrophenyl-beta-D-glucoside mutant W373K Zea mays
3.2.1.21 3.5
-
4-nitrophenyl-beta-D-glucoside mutant F200K Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Zea mays P49235
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-beta-D-glucoside + H2O
-
Zea mays 4-methylumbelliferone + beta-D-glucose
-
?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Zea mays 4-nitrophenol + D-glucopyranose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 ZM-p60.1
-
Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.22
-
4-nitrophenyl-beta-D-glucoside mutant F193A Zea mays
3.2.1.21 0.22
-
4-methylumbelliferyl-beta-D-glucoside mutant W373K Zea mays
3.2.1.21 0.63
-
4-nitrophenyl-beta-D-glucoside mutant W373K Zea mays
3.2.1.21 1.29
-
4-methylumbelliferyl-beta-D-glucoside mutant F193A Zea mays
3.2.1.21 1.87
-
4-methylumbelliferyl-beta-D-glucoside mutant F200K Zea mays
3.2.1.21 2.43
-
4-nitrophenyl-beta-D-glucoside mutant F200K Zea mays
3.2.1.21 42.8
-
4-nitrophenyl-beta-D-glucoside wild-type Zea mays
3.2.1.21 49.27
-
4-nitrophenyl-beta-D-glucoside mutant F461L Zea mays
3.2.1.21 53.8
-
4-methylumbelliferyl-beta-D-glucoside wild-type Zea mays
3.2.1.21 70.88
-
4-methylumbelliferyl-beta-D-glucoside mutant F461L Zea mays