EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.75 | dithiothreitol | 2fold stimulation at 5 mM | Sulfurisphaera tokodaii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.75 | expression in Escherichia coli | Sulfurisphaera tokodaii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.75 | iodoacetamide | - |
Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.75 | 0.025 | - |
NADPH | pH 7.8, 65°C | Sulfurisphaera tokodaii | |
1.2.1.75 | 0.04 | - |
malonyl-CoA | pH 7.8, 65°C | Sulfurisphaera tokodaii | |
1.2.1.75 | 0.1 | - |
malonyl-CoA | pH 7.8, 65°C | Metallosphaera sedula |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.75 | Mg2+ | 2fold stimulation at 5 mM | Sulfurisphaera tokodaii | |
1.2.1.75 | Mn2+ | 2fold stimulation at 5 mM | Sulfurisphaera tokodaii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.75 | 39000 | - |
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
1.2.1.75 | 39000 | - |
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
1.2.1.75 | 43000 | - |
4 * 43000, SDS-PAGE | Metallosphaera sedula |
1.2.1.75 | 45000 | - |
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
1.2.1.75 | 45000 | - |
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
1.2.1.75 | 150000 | - |
gel filtration | Metallosphaera sedula |
1.2.1.75 | 160000 | - |
gel filtration | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.75 | Metallosphaera sedula | - |
- |
- |
1.2.1.75 | Sulfurisphaera tokodaii | Q96YK1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.75 | partly | Metallosphaera sedula |
1.2.1.75 | recombinant protein | Sulfurisphaera tokodaii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.75 | 4.6 | - |
pH 7.8, 65°C | Metallosphaera sedula |
1.2.1.75 | 44 | - |
pH 7.8, 65°C | Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.75 | malonate semialdehyde + coenzyme A + NADP+ | - |
Sulfurisphaera tokodaii | malonyl-CoA + NADPH + H+ | - |
r | |
1.2.1.75 | malonyl-CoA + NADPH + H+ | - |
Metallosphaera sedula | malonate semialdehyde + coenzyme A + NADP+ | - |
? | |
1.2.1.75 | malonyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii | malonate semialdehyde + coenzyme A + NADP+ | - |
r | |
1.2.1.75 | succinate semialdehyde + coenzyme A + NADP+ | - |
Sulfurisphaera tokodaii | succinyl-CoA + NADPH + H+ | - |
r | |
1.2.1.75 | succinyl-CoA + NADPH + H+ | at 25% of the rate with malonyl-CoA | Sulfurisphaera tokodaii | succinate semialdehyde + coenzyme A + NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.75 | dimer | or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
1.2.1.75 | More | enzyme in its native state is associated with small RNA | Sulfurisphaera tokodaii |
1.2.1.75 | tetramer | 4 * 43000, SDS-PAGE | Metallosphaera sedula |
1.2.1.75 | tetramer | or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.75 | 2 | 8 | malonyl-CoA | per subunit, pH 7.8, 65°C | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.75 | 7.2 | - |
- |
Sulfurisphaera tokodaii |
1.2.1.75 | 7.8 | - |
- |
Metallosphaera sedula |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.75 | 6 | - |
half-maximal activity | Sulfurisphaera tokodaii |
1.2.1.75 | 6.5 | - |
half-maximal activity | Metallosphaera sedula |
1.2.1.75 | 8 | - |
half-maximal activity | Sulfurisphaera tokodaii |
1.2.1.75 | 8.5 | - |
half-maximal activity | Metallosphaera sedula |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.75 | coenzyme A | - |
Sulfurisphaera tokodaii | |
1.2.1.75 | additional information | no cofactor: NADH | Metallosphaera sedula | |
1.2.1.75 | additional information | no cofactor: NADH, phosphate | Sulfurisphaera tokodaii | |
1.2.1.75 | NADP+ | - |
Sulfurisphaera tokodaii | |
1.2.1.75 | NADPH | - |
Metallosphaera sedula | |
1.2.1.75 | NADPH | - |
Sulfurisphaera tokodaii |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.2.1.75 | 0.04 | - |
pH 7.8, 65°C | Sulfurisphaera tokodaii | iodoacetamide |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.2.1.75 | Metallosphaera sedula | in heterotropically grown cells, activity is downregulated to 40 nmol per min and mg | down |
1.2.1.75 | Metallosphaera sedula | under autotrophic conditions, 10fold upregulation | up |