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Literature summary extracted from

  • Alber, B.; Olinger, M.; Rieder, A.; Kockelkorn, D.; Jobst, B.; Hägler, M.; Fuchs, G.
    Malonyl-coenzyme A reductase in the modified 3-hydroxypropionate cycle for autotrophic carbon fixation in archaeal Metallosphaera and Sulfolobus spp (2006), J. Bacteriol., 188, 8551-8559.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.75 dithiothreitol 2fold stimulation at 5 mM Sulfurisphaera tokodaii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.75 expression in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.75 iodoacetamide
-
Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.75 0.025
-
NADPH pH 7.8, 65°C Sulfurisphaera tokodaii
1.2.1.75 0.04
-
malonyl-CoA pH 7.8, 65°C Sulfurisphaera tokodaii
1.2.1.75 0.1
-
malonyl-CoA pH 7.8, 65°C Metallosphaera sedula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.75 Mg2+ 2fold stimulation at 5 mM Sulfurisphaera tokodaii
1.2.1.75 Mn2+ 2fold stimulation at 5 mM Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.75 39000
-
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
1.2.1.75 39000
-
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
1.2.1.75 43000
-
4 * 43000, SDS-PAGE Metallosphaera sedula
1.2.1.75 45000
-
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
1.2.1.75 45000
-
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
1.2.1.75 150000
-
gel filtration Metallosphaera sedula
1.2.1.75 160000
-
gel filtration Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.75 Metallosphaera sedula
-
-
-
1.2.1.75 Sulfurisphaera tokodaii Q96YK1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.75 partly Metallosphaera sedula
1.2.1.75 recombinant protein Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.75 4.6
-
pH 7.8, 65°C Metallosphaera sedula
1.2.1.75 44
-
pH 7.8, 65°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.75 malonate semialdehyde + coenzyme A + NADP+
-
Sulfurisphaera tokodaii malonyl-CoA + NADPH + H+
-
r
1.2.1.75 malonyl-CoA + NADPH + H+
-
Metallosphaera sedula malonate semialdehyde + coenzyme A + NADP+
-
?
1.2.1.75 malonyl-CoA + NADPH + H+
-
Sulfurisphaera tokodaii malonate semialdehyde + coenzyme A + NADP+
-
r
1.2.1.75 succinate semialdehyde + coenzyme A + NADP+
-
Sulfurisphaera tokodaii succinyl-CoA + NADPH + H+
-
r
1.2.1.75 succinyl-CoA + NADPH + H+ at 25% of the rate with malonyl-CoA Sulfurisphaera tokodaii succinate semialdehyde + coenzyme A + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.2.1.75 dimer or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
1.2.1.75 More enzyme in its native state is associated with small RNA Sulfurisphaera tokodaii
1.2.1.75 tetramer 4 * 43000, SDS-PAGE Metallosphaera sedula
1.2.1.75 tetramer or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.75 2 8 malonyl-CoA per subunit, pH 7.8, 65°C Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.75 7.2
-
-
Sulfurisphaera tokodaii
1.2.1.75 7.8
-
-
Metallosphaera sedula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.75 6
-
half-maximal activity Sulfurisphaera tokodaii
1.2.1.75 6.5
-
half-maximal activity Metallosphaera sedula
1.2.1.75 8
-
half-maximal activity Sulfurisphaera tokodaii
1.2.1.75 8.5
-
half-maximal activity Metallosphaera sedula

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.75 coenzyme A
-
Sulfurisphaera tokodaii
1.2.1.75 additional information no cofactor: NADH Metallosphaera sedula
1.2.1.75 additional information no cofactor: NADH, phosphate Sulfurisphaera tokodaii
1.2.1.75 NADP+
-
Sulfurisphaera tokodaii
1.2.1.75 NADPH
-
Metallosphaera sedula
1.2.1.75 NADPH
-
Sulfurisphaera tokodaii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.1.75 0.04
-
pH 7.8, 65°C Sulfurisphaera tokodaii iodoacetamide

Expression

EC Number Organism Comment Expression
1.2.1.75 Metallosphaera sedula in heterotropically grown cells, activity is downregulated to 40 nmol per min and mg down
1.2.1.75 Metallosphaera sedula under autotrophic conditions, 10fold upregulation up