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Literature summary extracted from

  • Mayer, J.; Cook, A.M.
    Homotaurine metabolized to 3-sulfopropanoate in Cupriavidus necator H16: enzymes and genes in a patchwork pathway (2009), J. Bacteriol., 191, 6052-6058.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 4-aminobutyrate induction during growth with 4-aminobutyrate (GABA) Cupriavidus necator
2.6.1.19 4-aminobutyrate induction during growth with 4-aminobutyrate (GABA) Cupriavidus necator

General Stability

EC Number General Stability Organism
1.2.1.16 enzyme is labile (50% loss of activity overnight) but can be largely stabilized (in crude extract only) by the addition of 10 mM dithiothreitol 1.2.1.16 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.041
-
 3-sulfopropanal Km apparent value Cupriavidus necator
1.2.1.16 0.23
-
 NAD+ Km apparent value Cupriavidus necator
1.2.1.16 0.35
-
 NADP+ Km apparent value Cupriavidus necator
2.6.1.19 6.3
-
 2-oxoglutarate Km apparent value Cupriavidus necator
2.6.1.19 8.5
-
 3-Aminopropanesulfonate Km apparent value Cupriavidus necator
2.6.1.19 9.9
-
 4-aminobutyrate Km apparent value Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.16 49000
-
 SDS-PAGE Cupriavidus necator
2.3.3.15 63000
-
 by SDS-PAGE Cupriavidus necator
2.6.1.19 45000
-
 denaturated protein, determined by SDS-PAGE Cupriavidus necator
2.6.1.19 120000
-
 mass of native protein, determined by gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 3-sulfopropanal + NAD(P)+ + H2O Cupriavidus necator
-
 3-sulfopropanoate + NAD(P)H + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NAD(P)+ + H2O Cupriavidus necator
-
 succinate + NAD(P)H + 2 H+
-
 ?
2.3.3.15 2-sulfoacetaldehyde + phosphate Cupriavidus necator
-
 acetyl phosphate + sulfite
-
 ?
2.6.1.19 3-aminopropanesulfonate + 2-oxoglutarate Cupriavidus necator substrate is homotaurine 3-sulfopropanal + L-glutamate
-
 ?
2.6.1.19 4-aminobutyrate + 2-oxoglutarate Cupriavidus necator
-
 4-oxobutanoate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Cupriavidus necator Q0K2K1
-
-
2.3.3.15 Cupriavidus necator Q0K022
-
-
2.6.1.19 Cupriavidus necator Q0K2K2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 by ammonium sulfate precipitation and anion exchange chromatography and gel filtration Cupriavidus necator
2.6.1.19 by anion exchange chromatography Cupriavidus necator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.16 cell culture
-
 Cupriavidus necator
-
2.3.3.15 cell culture
-
 Cupriavidus necator
-
2.6.1.19 cell culture
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 additional information
-
 specific activity of 0.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is sulfopropanal and NADP+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 0.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 0.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 0.6 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is sulfopropanal and NAD+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 0.7 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 10 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is sulfopropanal and NADP+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 2.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is sulfopropanal and NADP+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is sulfopropanal and NAD+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is sulfopropanal and NAD+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 5.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 7.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ Cupriavidus necator
1.2.1.16 additional information
-
 specific activity of 7.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ Cupriavidus necator
2.6.1.19 additional information
-
 specific activity is 52% with oxaloacetate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity is 59% with glyoxalate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity is 63% with pyruvate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity is 9% with 2-oxobutyrate as amino group acceptor (normalized to that with 2-oxoglutarate (100%: 46 mkat/kg protein)), the donor is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 0.02 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 0.03 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is 4-aminobutyrate Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 6.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 8.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinate semialdehyde Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 8.2 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is 4-aminobutyrate Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 8.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is homotaurine Cupriavidus necator
2.6.1.19 additional information
-
 specific activity of 9.9 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is 4-aminobutyrate Cupriavidus necator
2.6.1.19 additional information
-
 specific activity with 3-aminopropanoate, taurine, (S)-cysteate, 3-aminopropanol or 4-aminobutanol as substrates is not detected Cupriavidus necator
2.6.1.19 additional information
-
 specific activity, normalized to that with homotaurine (100%: 271 mkat/kg protein), is 226% with 5-aminovalerate 6-aminocapronate as substrate Cupriavidus necator
2.6.1.19 additional information
-
 specific activity, normalized to that with homotaurine (100%: 271 mkat/kg protein), is 25% with 6-aminocapronate as substrate Cupriavidus necator
2.6.1.19 additional information
-
 specific activity, normalized to that with homotaurine (100%: 271 mkat/kg protein), is 270% with 4-aminobutyrate as substrate Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 3-sulfopropanal + NAD(P)+ + H2O
-
 Cupriavidus necator 3-sulfopropanoate + NAD(P)H + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NAD(P)+ + H2O
-
 Cupriavidus necator succinate + NAD(P)H + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Cupriavidus necator succinate + NADH + H+
-
 r
2.3.3.15 2-sulfoacetaldehyde + phosphate
-
 Cupriavidus necator acetyl phosphate + sulfite
-
 ?
2.6.1.19 3-aminopropanesulfonate + 2-oxoglutarate substrate is homotaurine Cupriavidus necator 3-sulfopropanal + L-glutamate
-
 ?
2.6.1.19 4-aminobutyrate + 2-oxoglutarate
-
 Cupriavidus necator 4-oxobutanoate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.16 homodimer
-
 Cupriavidus necator
2.6.1.19 homodimer gel filtration Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 3-sulfopropanal dehydrogenase
-
 Cupriavidus necator
1.2.1.16 GabD1
-
 Cupriavidus necator
1.2.1.16 NAD(P)+-coupled 3-sulfopropanal dehydrogenase
-
 Cupriavidus necator
1.2.1.16 succinate-semialdehyde dehydrogenase
-
 Cupriavidus necator
1.2.1.16 sulfopropanal dehydrogenase
-
 Cupriavidus necator
2.3.3.15 sulfoacetaldehyde acetyltransferase
-
 Cupriavidus necator
2.3.3.15 Xsc
-
 Cupriavidus necator
2.6.1.19 aminobutyrate transaminase
-
 Cupriavidus necator
2.6.1.19 GABA transaminase
-
 Cupriavidus necator
2.6.1.19 GABA:2-oxoglutarate aminotransferase
-
 Cupriavidus necator
2.6.1.19 GabT
-
 Cupriavidus necator
2.6.1.19 homotaurine:2-oxoglutarate aminotransferase
-
 Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.19 9
-
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.16 8 10.5 specific activity increases steadily to a broad optimum Cupriavidus necator
2.6.1.19 7.2 10 enzyme activity is detected Cupriavidus necator

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.16 4.5
-
 enzyme is almost inactive Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.19 pyridoxal 5'-phosphate cofactor remains bound during purification Cupriavidus necator

Expression

EC Number Organism Comment Expression
1.2.1.16 Cupriavidus necator transcript is detected inducibly in both homotaurine and GABA-grown cells up
2.6.1.19 Cupriavidus necator transcript is detected inducibly in both homotaurine and GABA-grown cells up

General Information

EC Number General Information Comment Organism
1.2.1.16 metabolism GABA metabolism Cupriavidus necator
2.3.3.15 metabolism homotaurine metabolism Cupriavidus necator
2.6.1.19 metabolism GABA metabolism Cupriavidus necator