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Literature summary extracted from

  • Kreimeyer, A.; Perret, A.; Lechaplais, C.; Vallenet, D.; Medigue, C.; Salanoubat, M.; Weissenbach, J.
    Identification of the last unknown genes in the fermentation pathway of lysine (2007), J. Biol. Chem., 282, 7191-7197 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.B2 expressed in Escherichia coli BL21 DE3 cells unidentified

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.B2 NADPH the enzyme is 65% inhibited by the addition of 0.1 mM NADPH unidentified

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.B2 0.0593
-
 NADP+ in the presence of 0.75 mM L-erythro-3,5-diaminohexanoate unidentified
1.4.1.B2 0.1978
-
 L-erythro-3,5-diaminohexanoate in the presence of 1 mM NADP+ unidentified
1.4.1.B2 3.2
-
 L-erythro-3,5-diaminohexanoate in the presence of 30 mM NAD+ unidentified
1.4.1.B2 16.5
-
 NAD+ in the presence of 15 mM L-erythro-3,5-diaminohexanoate unidentified

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.B2 39102
-
 2 * 39102, calculated from sequence unidentified
1.4.1.B2 40000
-
 2 * 40000, SDS-PAGE unidentified

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.B2 L-erythro-3,5-diaminohexanoate + H2O + NADP+ unidentified
-
 (S)-5-amino-3-oxohexanoate + NH3 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.B2 unidentified A1X0G6 wastewater metagenomic collection
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.B2 recombinant enzyme, HisTrap FF column chromatography, MonoQ column chromatograhy, and Superdex 200 gel filtration unidentified

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.B2 L-erythro-3,5-diaminohexanoate + H2O + NAD+
-
 unidentified 5-amino-3-oxohexanoate + NH3 + NADH + H+
-
 ?
1.4.1.B2 L-erythro-3,5-diaminohexanoate + H2O + NADP+
-
 unidentified (S)-5-amino-3-oxohexanoate + NH3 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.B2 dimer 2 * 40000, SDS-PAGE unidentified
1.4.1.B2 dimer 2 * 39102, calculated from sequence unidentified

Synonyms

EC Number Synonyms Comment Organism
1.4.1.B2 Kdd
-
 unidentified

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.B2 NAD+ Vmax/Km for NADP+ is 223fold higher than Vmax/Km for NAD+ unidentified
1.4.1.B2 NADP+ Vmax/Km for NADP+ is 223fold higher than Vmax/Km for NAD+ unidentified